文章摘要
董靖德,靳 凌,张 丽,石静萍,王小姗.厄贝沙坦对大鼠脑缺血再灌注后NF-κB和IκBα的影响[J].南京医科大学学报,2012,(8):1079~1082
厄贝沙坦对大鼠脑缺血再灌注后NF-κB和IκBα的影响
Effect of Irbesartan on NF-κB and IκBα in the ischemic brain of ischemia-reperfusion rat
投稿时间:2011-12-21  
DOI:10.7655
中文关键词: 脑缺血  厄贝沙坦  NF-κB  IκBα
英文关键词: cerebral ischemia  Irbesartan  NF-κB  IκBα
基金项目:南京医科大学科技发展基金(08NMUM114)
作者单位
董靖德 南京医科大学附属脑科医院老年神经科,江苏 南京 210029 
靳 凌 南京医科大学附属脑科医院老年神经科,江苏 南京 210029 
张 丽 南京医科大学附属脑科医院老年神经科,江苏 南京 210029 
石静萍 南京医科大学附属脑科医院神经科,江苏 南京 210029 
王小姗 南京医科大学附属脑科医院神经科,江苏 南京 210029 
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中文摘要:
      目的:观察厄贝沙坦对大鼠脑缺血再灌注后缺血区炎症因子的影响?方法:将健康的雄性SD大鼠随机分为3组,即假手术组?缺血再灌注(I/R)组?厄贝沙坦预处理组?厄贝沙坦预处理组在制备模型前给予厄贝沙坦30 mg/(kg·d)干预3周,采用线栓法制备大鼠大脑中动脉缺血再灌注模型?脑缺血90 min再灌注24?72 h后用Longa标准进行神经功能评分,用Western blot法测大鼠脑缺血组织内NF-κB p65?IκBα的表达水平?结果:①与I/R组相比较,厄贝沙坦组神经功能缺损程度评分明显改善(P < 0.05);②脑缺血再灌注后24及72 h,I/R组缺血区NF-κB p65的表达明显高于假手术组(P < 0.01),与I/R组相比较,厄贝沙坦预干预组缺血区NF-κB p65的表达明显降低(P < 0.05);③脑缺血再灌注后24 h,I/R组缺血区IκBα的表达高于假手术组(P < 0.05),与I/R组相比较,厄贝沙坦预干预组缺血区IκBα的表达明显增高(P < 0.05)?结论:厄贝沙坦可通过提高IκBα的表达,抑制NF-κB的表达,改善大鼠局灶性脑缺血再灌注后神经功能缺损,对脑缺血再灌注起保护作用?
英文摘要:
      Objective:To observe the effect of Irbesartan on inflammatory factors in the brain of ischemia-reperfusion rat. Methods:The male SD rats were randomly assigned to sham operated group,ischemia-reperfusion(I/R) group and Irbsartan pretreatment group. The focal I/R model was made by suture occlusion of right middle cerebral artery(MCAO) for 90 min followed by suture retreat which was defined as the beginning of reperfusion. Irbesartan[30 mg/(kg·d)] was administered for 3 weeks before MCAO. After 24 h or 72 h of reperfusion,the neurologic function impairment was evaluated by Longa standard,NF-κB p65 and IκBα protein levels were examined by Western blot technique. Results:① Compared with I/R group,pretreatment with Irbsartan could significantly improve neurologic function (P < 0.05). ② Compared with the sham group,the NF-κB p65 protein was significantly increased after either 24 h or 72 h of reperfusion in I/R group(P < 0.01). Compared with I/R group,however,the NF-κB p65 protein was significantly decreased in Irbsartan pretreatment group(P < 0.05).③ Compared with the sham group,the IκBα protein was significantly increased after 24 h of reperfusion in I/R group(P < 0.05). Compared with I/R group,the IκBα protein was significantly increased in Irbsartan pretreatment group(P < 0.05). Conclusion:Irbesartan can protect brain and may improve the rat neurologic function through enhancing IκBα protein expression and inhibiting NF-κB protein expression in rat brain with ischemia-reperfusion injury.
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