文章摘要
赵凌云,刘南君,许永杰,李学英,刘晓红.一种适用于初试者的STZ法建T1DM大鼠模型的技术方案[J].南京医科大学学报,2012,(10):1480~1482
一种适用于初试者的STZ法建T1DM大鼠模型的技术方案
A new and suitable technical scheme of establishing type 1 diabetes (T1DM) rats model by STZ injection for beginners
投稿时间:2012-04-19  
DOI:10.7655
中文关键词: 链脲佐菌素  糖尿病  动物模型  Wistar大鼠
英文关键词: sreptozotocin (STZ)  diabetes mellitus  animal models  Wistar rats
基金项目:贵州省教育厅基金资助项目(2004213)﹔贵州省卫生厅基金资助项目(2004)
作者单位
赵凌云 遵义医学院细胞生物学与遗传学教研室,贵州 遵义 563003 
刘南君 遵义医学院细胞生物学与遗传学教研室,贵州 遵义 563003 
许永杰 遵义医学院细胞生物学与遗传学教研室,贵州 遵义 563003 
李学英 遵义医学院细胞生物学与遗传学教研室,贵州 遵义 563003 
刘晓红 遵义医学院生理学教研室,贵州 遵义 563003 
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中文摘要:
      目的:探索一种适用于初学者的以STZ法复制1型糖尿病(T1DM)大鼠模型的技术方案?方法:100只雄性Wistar大鼠随机分为模型组(DMM)和对照组(NC)?采用有别于现有文献报道的技术方案即分批次建模?DMM组分3批依次分别一次性腹腔注射60 mg/kg(DMM1)?70 mg/kg(DMM2)?55 mg/kg(DMM3)STZ溶液,NC组注射等量的柠檬酸盐缓冲液?DMM1?DMM2建模未成功者均编为DMM3?将72 h后禁食17 h空腹血糖值(FPG)≥11.1 mmol/L和相应生理指标作为成模的判断依据?结果:DMM1建模前后FPG无差异(P > 0.05),DMM1建模失败;DMM2建模前后FPG具有显著差异(P < 0.05),成模率为75%?DMM3补充剂量后建模成功?结论:分批次进行的STZ法建模方案适宜于初学者使用?
英文摘要:
      Objective:To explore a new and suitable technical scheme of establishing type 1 diabetes (T1DM) rats model by STZ injection for beginners. Methods:Total 100 male Wistar rats were divided into diabetes mellitus model (DMM) group and the control group (NC) randomly. The technical scheme for establishing animal model is different from the existing literature. The DMM group of two batches were followed by a single intraperitoneal injection 60 mg/kg(DMM1) or 70 mg/kg(DMM2) STZ solution respectively. the NC group was injected with the same amount of citrate buffer. The rats in DMM1 and DMM2 goups,which didn’t develop T1DM,were compiled into DMM3 group(55 mg/kg STZ by a intraperitoneal injection). After 72 h of STZ treatment,the rats were considerd as T1DM model with fasting plasma glucose (FPG) after 17 h fasting≥ 11.1 mmol /L and with corresponding physiological indicators. Results:The rat model can’t be constructed successfully by a single intraperitoneal injection STZ of 60 mg/kg. The FPG of rats in DMM2 group changed significantly before and after STZ injection(P < 0.05),and 75% of rats developed T1DM. The rats that didn’t develop T1DM with first injection of STZ developed T1DM after supplementary dose in DMM3 group. Conclusion:Constructing T1DM rats induced by STZ injection in batch is best for beginners to use.
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