文章摘要
焦雪花,马向华,卢 姗,田 婷,张媛媛.胃旁路术对非肥胖2型糖尿病大鼠肝脏葡萄糖激酶表达的影响[J].南京医科大学学报,2013,(8):1066~1069
胃旁路术对非肥胖2型糖尿病大鼠肝脏葡萄糖激酶表达的影响
Effect of gastric bypass on glucokinase expression in non-obese type 2 diabetid rat liver
投稿时间:2012-12-05  
DOI:10.7655/NYDXBNS20130808
中文关键词: 胃旁路术  2型糖尿病  GK大鼠  胰岛素  葡萄糖激酶
英文关键词: gastric bypass  type 2 diabetes mellitus  Goto-Kakizaki rats  insulin  glucokinase
基金项目:江苏省卫生厅医学科技发展基金(H201202)
作者单位
焦雪花 南京医科大学第一附属医院内分泌科,江苏 南京 210029吴江第一人民医院内分泌科,江苏 吴江 215200 
马向华 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
卢 姗 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
田 婷 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
张媛媛 南京医科大学第一附属医院内分泌科,江苏 南京 210029 
摘要点击次数: 1025
全文下载次数: 575
中文摘要:
      目的:探讨胃旁路术对非肥胖2型糖尿病GK大鼠肝脏葡萄糖激酶(glucokinase,GCK)表达的影响。方法:雄性GK和Wistar大鼠各20只,随机分为GK手术组(GO组)?GK假手术组(GS组)?Wistar手术组(WO组)和Wistar假手术组(WS组),每组10只。检测术前及术后第1?4?8周各组空腹血糖和血清胰岛素水平,术后8周取大鼠肝脏组织,采用RT-PCR检测肝脏GCK mRNA表达水平。结果:GO组术后第1周空腹血糖已开始下降,术后第1?4?8周分别由术前(8.73 ± 1.30)mmol/L下降到(6.84 ± 0.89)?(6.27 ± 0.93)?(5.86 ± 0.57)mmol/L(P < 0.05);随访过程中,GO组空腹血清胰岛素较术前和相应时间点GS组均有所升高,尚未达统计学差异(P > 0.05);GO组术后第8周肝脏GCK mRNA表达水平明显高于GS组(P < 0.05)。结论:胃旁路术能显著改善非肥胖2型糖尿病大鼠的糖代谢,手术的降糖作用可能与肝脏葡萄糖激酶表达增加有关。
英文摘要:
      Objective:To investigate the effect of gastric bypass on the expression of liver glucokinase(GCK) in non-obese Goto-Kakizaki (GK) type 2 diabetic rats. Methods:Twenty male GK rats and twenty male Wistar rats were randomly divided into four groups:GK operation group(GO group),GK sham operation group(GS group),Wistar operation group(WO group),Wistar sham operation group(WS group). There were 10 rats in each group. The fasting blood glucose and serum insulin levels were measured before operation and on the 1st,4th and 8th week after the operation. Liver tissues were harvested 8 weeks postoperatively and reverse transcriptase polymerase chain reaction was used to detect liver GCK mRNA expression after the operation. Results:The fasting blood glucose of GO group decreased as early as the 1st week after surgery,which decreased from preoperative (8.73 ± 1.30) mmol/L to (6.84 ± 0.89),(6.27 ± 0.93),(5.86 ± 0.57) mmol/L on the 1st,4th and 8th week postoperation (P < 0.05). The insulin level remained consistently higher in GO group with respect to GS group,but there was no significant change during the follow-up period. On the 8th week after surgery,the liver GCK mRNA level in GO group were significantly higher than GS group(P < 0.05). Conclusion:Gastric bypass can directly improve glucose metabolism in non-obese type 2 diabetic rats,the increased expression of liver tissue GCK mRNA might contribute to the hypoglycemic effect of the surgery.
查看全文   查看/发表评论  下载PDF阅读器
关闭