文章摘要
陈宜刚,许 蕊.褪黑素抑制缺氧引起的SGC-7901人胃癌细胞上皮-间充质转化[J].南京医科大学学报,2013,(11):1508~1513
褪黑素抑制缺氧引起的SGC-7901人胃癌细胞上皮-间充质转化
Melatonin inhibits hypoxia induced epithelial- mesenchymal transition of SGC-7901 gastric cancer cells
投稿时间:2013-06-03  
DOI:10.7655/NYDXBNS20131105
中文关键词: 缺氧  SGC-7901人胃癌细胞  上皮-间充质转化  褪黑素
英文关键词: hypoxia  SGC-7901 gastric cancer cells  EMT  melatonin
基金项目:国家自然科学基金青年基金(81201614)
作者单位
陈宜刚 泰州职业技术学院医学技术学院,江苏 泰州 225300 
许 蕊 南京医科大学生理学系,江苏 南京 210029 
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中文摘要:
      目的:研究褪黑素对缺氧引起的SGC-7901人胃癌细胞上皮-间充质转化(EMT)的作用,并初步揭示其分子机制。方法:在建立缺氧诱导SGC-7901人胃癌细胞发生EMT的基础上,通过免疫印迹方法检测蛋白表达水平?免疫荧光的方法检测蛋白在细胞中的分布和表达,研究褪黑素对EMT过程的作用。结果:缺氧使SGC-7901人胃癌细胞发生EMT,表现为形态发生改变,上皮标志物表达量减少?间充质标志物表达增加,褪黑素可抑制缺氧条件下活性氧簇(ROS)的产生和缺氧诱导因子1α(HIF-1α)的表达及EMT。结论:在缺氧条件下SGC-7901人胃癌细胞可发生EMT,褪黑素可抑制该过程。这些结果揭示了褪黑素在缺氧引起的肿瘤细胞EMT过程中发挥的抑制作用,为褪黑素的临床应用提供了重要的理论依据。
英文摘要:
      Objective:To elucidate the function of melatonin on hypoxia -induced epithelial- mesenchymal transition (EMT) of SGC-7901 gastric cancer cells and to reveal the mechanisms underlying. Methods:After hypoxia for 96h,expression of epithelial and mesenchymal makers of SGC-7901 gastric cancer cells was detected using Western blot and immunofluorescence was employed to investigate distribution of proteins to see if EMT acured. Then we treated SGC-7901 gastric cancer cells by 1 mmol/L concentration of melatonin under hypoxia environment to see if EMT was inhibited. Results:Hypoxia induced morphological change of SGC-7901 gastric cancer cells,as well as decreased E-cadherin and increased vimnetin and α-SMA. The reactive oxygen species ( ROS) and exprssion of hypoxia-inducible factor-1α(HIF-1α) both abolished by treatment of melatonin,so as EMT. Conclusion:We found that melatonin can abolish hypoxia-induced EMT of SGC-7901 gastric cancer cells,which can be used for clinical application.
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