文章摘要
胡兆兴,王维东,范军届,凡 进,金正帅.内源性甲状旁腺激素促进小鼠骨折愈合[J].南京医科大学学报,2014,(4):457~461
内源性甲状旁腺激素促进小鼠骨折愈合
Endogenous parathyroid hormone promotes fracture healing in mice
投稿时间:2013-12-11  
DOI:10.7655/NYDXBNS20140411
中文关键词: 甲状旁腺激素  基因敲除  骨折愈合  小动物CT
英文关键词: parathyroid hormone  gene knockout  fracture healing  micro-CT
基金项目:国家自然科学基金(81271988)
作者单位
胡兆兴 南京医科大学第一附属医院骨科,江苏南京210029 
王维东 南京医科大学第一附属医院骨科,江苏南京210029 
范军届 南京医科大学第一附属医院骨科,江苏南京210029 
凡 进 南京医科大学第一附属医院骨科,江苏南京210029 
金正帅 南京医科大学第一附属医院骨科,江苏南京210029 
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中文摘要:
      目的:探讨甲状旁腺激素(parathyroid hormone,PTH)在小鼠骨折愈合过程中的作用?方法:选择8周龄野生型和PTH基因敲除雄性小鼠各15只,制备右侧股骨开放性骨折模型,并于骨折后7?14?21 d处死,运用小动物显微CT及其相关软件测量骨痂区骨密度;运用组织学?免疫组织化学?X线?CT三维重建比较分析两组小鼠的术后骨折愈合情况?结果:术后14 d和21 d PTH基因敲除小鼠的骨折端骨密度显著低于PTH野生型组(P < 0.05)?X线和显微CT显示PTH基因敲除小鼠骨折愈合?骨痂的塑形和PTH野生型小鼠相比均延迟?结论:内源性PTH促进小鼠骨折愈合?
英文摘要:
      Objective:To determine the role of the parathyroid hormone (PTH) in fracture healing of mice. Methods:A total of 30 male mice at 8 weeks old (including 15 wild-type mice and 15 PTH gene knockout mice) were selected to establish right femur fracture model. At 7,14 and 21 days after the operation,the mice were sacrificed for measurement of bone mineral density (BMD) of the callus by micro-CT. To determine the role of PTH in fracture healing,the fracture models were assessed by histology,immunohistochemistry,X ray and micro-CT. Results:At 14 and 21 days after the operation,BMD in PTH gene knockout mice was significantly reduced compared to that in PTH wild-type mice (P < 0.05). X ray and micro-CT scanning showed that the fracture healing and the callus transformation and remodeling in PTH gene knockout mice were both delayed compared to those in wild-type mice. Conclusion:This study suggests that endogenous PTH promotes the fracture healing in mice.
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