文章摘要
胡 帅,俞 悦,高 云,吕 凌,李国强.IMP3与IGF2在乙肝相关性肝癌组织中表达及与临床病理特征的关系[J].南京医科大学学报,2014,(5):568~573
IMP3与IGF2在乙肝相关性肝癌组织中表达及与临床病理特征的关系
Expression of IMP3 and IGF2 in hepatitis B-virus associated hepatocelluar carcinoma and the relationship with clinicopathological features
投稿时间:2013-11-17  
DOI:10.7655/NYDXBNS20140507
中文关键词: IMP3  IGF2  乙肝相关性肝癌  临床病理特征
英文关键词: IMP3  IGF2  HBV-HCC  clinicopathological characteristics
基金项目:江苏省兴卫工程重点人才(RC201152);江苏省六大人才高峰项目(2013)
作者单位
胡 帅 南京医科大学第一附属医院肝脏移植中心,江苏 南京 210029 
俞 悦 南京医科大学第一附属医院肝脏移植中心,江苏 南京 210029 
高 云 南京医科大学第一附属医院肝脏移植中心,江苏 南京 210029 
吕 凌 南京医科大学第一附属医院肝脏移植中心,江苏 南京 210029 
李国强 南京医科大学第一附属医院肝脏移植中心,江苏 南京 210029
江苏盛泽医院普外科,江苏 苏州 215226 
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中文摘要:
      目的:研究胰岛素样生长因子2 mRNA结合蛋白3(IMP3)与胰岛素样生长因子2(IGF2)在乙肝相关性肝癌中的表达,并探讨二者的相关性及与乙肝相关性肝癌临床病理特征的关系?方法:采用免疫组织化学法,检测82例乙肝相关性肝癌组织及对应癌旁组织中IMP3及IGF2的表达;采用实时定量聚合酶链式反应法检测32例冰冻肝癌组织中IMP3及IGF2 mRNA含量;采用蛋白质印迹法检测4例IMP3强阳性染色及4例IMP3阴性染色对应的新鲜冰冻肝癌组织中IMP3及IGF2蛋白含量?结果:IMP3在62.2%肝癌组织中表达,在癌旁组织中不表达?IGF2在59.8%肝癌组织及14.6%癌旁组织中表达?同时,IMP3与IGF2在乙肝相关性肝癌组织中表达密切相关(rn = 0.335,P = 0.002)?另外,IMP3高表达与Edmondson-Steiner 3~4级(P = 0.001)?TNM 3~4期(P = 0.001)?α-甲胎蛋白(AFP)> 200 ng/ml (P = 0.012)及伴有门脉癌栓的肿瘤(P = 0.018)均密切相关;与IMP3表达相似,IGF2高表达亦与Edmondson-Steiner 3~4级(P = 0.001)?TNM 3~4期(P < 0.001)及伴有门脉癌栓的肿瘤(P < 0.001)密切相关?结论:IMP3与IGF2在乙肝相关性肝癌组织中表达密切相关,同时,IMP3与IGF2蛋白分子的检测有助于判断肿瘤恶性分化程度及进展?
英文摘要:
      Objective:To investigate the expression of insulin-like growth factor 2 mRNA binding protein 3 (IMP3) and insulin-like growth factor 2 (IGF2) in hepatitis B-virus associated hepatocelluar carcinoma (HBV-HCC) and study the correlation between IMP3 and IGF2 as well as their relationship with clinicopathological features of HBV-HCC. Methods:IMP3 and IGF2 were detected in 82 paraffin-embedded tumor tissues and corresponding adjacent benign tissues from HBV-HCC patients by immunohistochemical assay. IMP3 and IGF2 mRNA were detected in selected 32 freezing tissues by Real-time PCR. IMP3 and IGF2 proteins were detected in 4 strong positive and 4 negative immunohistochemical staining tissues by Western blot analysis. Results:IMP3 was expressed in 62.2% HBV-HCC tissues,but not detected in adjacent benign tissues. IGF2 was expressed in 59.8% HBV-HCC tissues and 14.6% adjacent benign tissues. Meanwhile,the expression of IMP3 was significantly correlated with IGF2 expression in HBV-HCC tissues (rn = 0.335,P = 0.002). Additionally,our results showed that IMP3 expression was significantly correlated with TNM 3-4 tumor stage (P = 0.001),Edmondson-Steiner 3-4 tumor grade (P = 0.001),Alpha Fetal Protein (AFP)> 200 ng/ml (P = 0.012) and portal vein tumor thrombus (P = 0.018). Similar to the expression of IMP3,IGF2 expression was significantly correlated with Edmondson-Steiner 3-4 tumor grade (P = 0.001),TNM 3-4 tumor stage (P < 0.001) and portal vein tumor thrombus (P < 0.001). Conclusion:Our results indicate that IMP3 was significantly correlated with IGF2 expression in HBV-HCC and detection of IMP3 and IGF2 was helpful to evaluate the differentiation and progression of HBV-HCC.
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