牛磺酸对氧化应激损伤的神经保护作用
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Neuroprotection of taurine induced by hydrogen peroxide
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    摘要:

    目的:探讨牛磺酸对过氧化氢(H2O2)引起的神经元损伤的保护作用-方法:采用不同浓度的H2O2处理神经元,制备体外氧化应激模型,造模前24 h分别给予5-15-25 mmol/L牛磺酸预处理-采用特异性荧光探针DCFH-DA检测神经元活性氧自由基水平,检测乳酸脱氢酶(LDH)释放率反映神经元损伤程度,用免疫印迹法检测脑源性营养因子(brain-derived neurotrophic factor,BDNF)及突触相关蛋白的表达水平-结果:与Control组比较,不同浓度的H2O2(25-50-100 μmol/L)均能使LDH向胞外释放增多(P < 0.05),15-25 mmol/L的牛磺酸均能减少H2O2引起的LDH的释放(P < 0.05)-100 μmol/L H2O2使神经元胞内活性氧水平升高,仅25 mmol/L的牛磺酸能抑制H2O2诱导的胞内活性氧的增多(P < 0.05)-100 μmol/L H2O2处理神经元导致BDNF-突触相关蛋白Synapsin和Spinophilin表达水平下降,给予25 mmol/L的牛磺酸能部分逆转H2O2诱导的蛋白水平的下降(P < 0.05)-结论:牛磺酸能够减轻H2O2引起的神经元损伤,具有较好的神经保护作用-

    Abstract:

    Objective:To explore the protective effect of taurine on injured neurons induced by hydrogen peroxide (H2O2). Methods:Neurons were treated with different doses of H2O2. Oxidative stress model was prepared in vitro. Taurine was applied at 5,15 or 25 mmol/L 24 h prior to H2O2 treatment. Specific fluorescent probe DCFH-DA was performed to analyze reactive oxygen species level of neurons. Lactic dehydrogenase (LDH) release assay was performed to detect the injury degree of neurons. Brain-derived neurotrophic factor (BDNF) and synaptic-related protein levels were evaluated by Western blotting. Results:Compared with the control group,different doses of H2O2 (5,50 and 100 μmol/L) increased LDH release to the extracellular medium (P < 0.05),while 15 and 25 mmol/L taurine decreased the release (P < 0.05). One hundred μmol/L H2O2 led to an increase of reactive oxygen species level and decreased BDNF,synapsin and spinophilin protein expressions (P < 0.05). After treatment with 25 mmol/L taurine,the above indicators were attenuated (P < 0.05). Conclusion:Taurine can alleviate H2O2 induced neuron damages and show neuroprotective effect against oxidative stress.

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高丽艳,朱东亚.牛磺酸对氧化应激损伤的神经保护作用[J].南京医科大学学报(自然科学版),2014,(8):1072-1076

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  • 收稿日期:2014-01-14
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  • 在线发布日期: 2014-08-03
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