Objective:To analyze laboratory diagnosis coincidence rate of hepatitis B in Jiangsu Province in 2012. Methods:Stratified random method was used to chose 2-3 cities from each of south,middle and north of Jiangsu Province,and further chose the provincial,municipal and county hospitals from each city. Results:The laboratory diagnosis coincidence rate was 75.26% based on effective 287 cases from a total of 23 county-level or above medical institutions. The diagnostic accordance rate in the provincial hospitals was the lowest. The awareness rates of laboratory diagnostic criteria and report type of HBV for clinicians were 23.38% and 36.52%,respectively,the highest of which was from country hospitals. Conclusion:The laboratory diagnosis coincidence rate of hepatitis B in Jiangsu Province is relative high,however,the awareness rate about HBV relevant knowledge needs to be improved. Therefore,it is necessary to strengthen the training and communication,improve the HBV monitoring network as well as propel practical HBV diagnostic criteria.