文章摘要
冯小可,刘 皓,冷秀梅,张前德,谢立群.基于气相色谱质谱联用仪的糖尿病胃轻瘫大鼠血清代谢组学研究[J].南京医科大学学报,2015,(6):793~797
基于气相色谱质谱联用仪的糖尿病胃轻瘫大鼠血清代谢组学研究
The high performance liquid chromatography study of serum from diabetic gastroparesis’rats by gas chromatograph mass spectrometer equipment
投稿时间:2015-01-19  
DOI:10.7655/NYDXBNS20150607
中文关键词: 糖尿病胃轻瘫  代谢组学  气相色谱和质谱联用仪
英文关键词: diabetic gastroparesis  metabonomics  gas chromatographic mass spectrometric analysis
基金项目:江苏省高校自然科学基金面上项目(12KJB360002);第二批江苏省优秀中青年中医临床人才研修专项课题(YX1215)
作者单位
冯小可 南京医科大学第一附属医院中医科,江苏 南京 210029
南京医科大学中西医结合研究所,江苏 南京 210029 
刘 皓 南京医科大学第一附属医院中医科,江苏 南京 210029
南京医科大学中西医结合研究所,江苏 南京 210029 
冷秀梅 南京医科大学第一附属医院中医科,江苏 南京 210029
南京医科大学中西医结合研究所,江苏 南京 210029 
张前德 南京医科大学中西医结合研究所,江苏 南京 210029 
谢立群 南京医科大学第一附属医院中医科,江苏 南京 210029
南京医科大学中西医结合研究所,江苏 南京 210029 
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中文摘要:
      目的:分析2型糖尿病胃轻瘫组大鼠与正常组大鼠血清分子代谢物代谢轮廓的变化。方法:应用气相色谱和质谱联用仪方法,通过偏最小二乘法-判别分析方法(partial least squares linear discriminant analysis,PLS-DA)和正交偏最小二乘法-判别分析方法(orthogonal partial least squares linear discriminant analysis,OPLS-DA),鉴别糖尿病胃羟瘫组和正常组间血清小分子代谢物的区别。结果:两组血清的多种氨基酸成分有显著差异,与正常组比较,糖尿病胃轻瘫组苏氨酸?丙氨酸?甘氨酸?肌酸及苯丙氨酸含量明显下降,尿酸含量明显上升。结论:甘氨酸?肌酸?苯丙氨酸等多种氨基酸含量的变化可能与糖尿病胃轻瘫的发病机制有关。
英文摘要:
      Objective:To analyze the changes of serum molecule metabolites between rats with diabetic gastroparesis and healthy rats. Methods: Partial least squares linear discriminant analysis (PLS-DA) and orthogonal partial least squares linear discriminant analysis (OPLS-DA) were used to identify the differences of two groups on the basis of high performance liquid chromatography. Results: There were statistically significant differences in the amino acids between serum of the two groups, and compared with the normal control group, the contents of threonine, lactamine, aminoacetic acid, creatine and phenylalanine in the diabetic gastroparesis group were significantly decreased, however, the content of .trioxypurine was significantly increased. Conclusion: The results demonstrated that the different amino acids,which included glycocine/creatine/phenylalanine and so on,may be related to the pathogenesis of diabetic gastroparesis. Therefore, it has important forecasting significance to make use of gas chromatographic mass spectrometric analysis to study the pathogenesis of diabetic gastroparesis.
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