文章摘要
彭秋月,宣文华,石雨濛,谈文峰,王 芳,张前德.白细胞介素29抑制破骨细胞分化的研究[J].南京医科大学学报,2016,(3):313~317
白细胞介素29抑制破骨细胞分化的研究
Inhibitory effect of interleukin 29 on osteoclast differentiation
投稿时间:2015-10-19  
DOI:10.7655/NYDXBNS20160312
中文关键词: 白细胞介素29  破骨细胞  类风湿关节炎
英文关键词: interleukin 29  osteoclast  rheumatoid arthritis
基金项目:国家自然科学基金(81471610,81471611)
作者单位
彭秋月 南京医科大学中西医结合研究所,江苏 南京 210029 
宣文华 南京医科大学第一附属医院风湿免疫科,江苏 南京 210029 
石雨濛 南京医科大学第一附属医院风湿免疫科,江苏 南京 210029 
谈文峰 南京医科大学第一附属医院风湿免疫科,江苏 南京 210029 
王 芳 南京医科大学第一附属医院心血管内科,江苏 南京 210029 
张前德 南京医科大学中西医结合研究所,江苏 南京 210029 
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中文摘要:
      目的:新型细胞因子白细胞介素(interleukin,IL)-29具有抗病毒?抗肿瘤?免疫调节等作用,但其在破骨细胞分化中的作用尚未见报道。本研究探讨IL-29对核因子κB受体活化因子配基(receptor activation of nuclear-κB ligand,RANKL)诱导小鼠巨噬细胞RAW264.7(破骨前体细胞)向破骨细胞分化的作用。方法:流式检测IL-29特异性受体IL-28Rα在RAW264.7细胞表面的表达,CCK8法检测IL-29对RAW264.7细胞增殖的影响。不同浓度IL-29重组因子加入到RANKL诱导的RAW264.7向破骨细胞分化的体系中,5 d后通过抗酒石酸酸性磷酸酶染色检测IL-29对破骨细胞数目的影响。实时荧光定量PCR检测IL-29对破骨细胞标志性基因TRAP?CTR?CTSK及相关基因TRAF6?RANK表达的影响。结果:IL-29呈剂量依赖性抑制RANKL诱导的RAW264.7中破骨细胞的形成,100 ng/mL IL-29可显著抑制破骨细胞的形成以及破骨细胞形成相关基因TRAP?CTR?CTSK?TRAF6?RANK的表达。结论:本研究首次证实IL-29可以抑制破骨细胞形成及其功能。
英文摘要:
      Objective:The novel cytokine interleukin (IL)-29 has been reported to induce antiviral, antitumor, and immune responses. However,the effect of IL-29 on osteoclast differentiation remains unclear. This study aimed to explore the effect of IL-29 on osteoclast differentiation and function in RANKL-induced in RAW264.7 cells (osteoclast precursors). Methods:The expression of IL-28Rα (specific receptor of IL-29) on RAW264.7 cells was evaluated by flow cytometry. RAW264.7 cells were stimulated with RANKL and different doses of recombinant IL-29 for 5 d,then the osteoclast formation was evaluated by counting multinucleated cells for tartrate-resistant acid phosphatase (TRAP) staining. Furthermore,the effect of IL-29 on the RANKL-induced expression of osteoclastic genes (TRAP,CTR and CTSK) and relevant genes(TRAF6 and RANK) was detected by real time PCR. Results:IL-29 inhibited the osteoclast formation in a dose-dependent manner in RANKL-induced RAW264.7 cells,and the dose of 100 ng/ml IL-29 significantly reduced the number of TRAP positive multinucleated cells and mRNA expression of osteoclastic genes (TRAP,CTR and CTSK) and relevant genes(TRAF6 and RANK). Conclusion:The findings in the present study indicate,for the first time,that IL-29 could inhibit osteoclast formation and function.
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