文章摘要
冯雨苗,孙 澜,刘 标,杨 如,孙文洁,华 纲,陈朝潮,赵斌斌,施 辛.宫颈尖锐湿疣中hTERC基因扩增和CD105表达的意义[J].南京医科大学学报,2016,(8):979~982
宫颈尖锐湿疣中hTERC基因扩增和CD105表达的意义
The expression and significance of hTERC and CD105 in cervical candyloma acuminatum
投稿时间:2015-06-17  
DOI:10.7655/NYDXBNS20160817
中文关键词: 宫颈尖锐湿疣  宫颈上皮内瘤变  hTERC基因  CD105
英文关键词: cervical candyloma acuminatum  cervical intraepithelial neoplasia  hTERC gene  CD105
基金项目:南京医科大学校基金(2012NJMU162)
作者单位
冯雨苗 南京医科大学附属苏州医院皮肤科 
孙 澜 南京医科大学附属苏州医院皮肤科 
刘 标 病理科 
杨 如 妇产科,江苏 苏州 215001 
孙文洁 宜兴市人民医院皮肤科,江苏 宜兴 214200 
华 纲 南京医科大学附属苏州医院皮肤科 
陈朝潮 南京医科大学附属苏州医院皮肤科 
赵斌斌 妇产科,江苏 苏州 215001 
施 辛 苏州大学附属第二医院皮肤科,江苏 苏州 215004 
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中文摘要:
      目的:探讨hTERC基因扩增和CD105在宫颈尖锐湿疣(candyloma acuminatum,CA)中的表达及其临床意义?方法:分别采用免疫荧光原位杂交(FISH)和免疫组化方法检测宫颈CA组织中hTERC基因扩增和CD105的表达?67例宫颈CA中单纯性CA 31例,CA-CIN Ⅰ级19例,CA-CIN Ⅱ级11例,CA-CIN Ⅲ级6例;宫颈癌35例;正常宫颈黏膜24例作为对照?结果:宫颈正常黏膜?宫颈CA和宫颈癌中hTERC基因扩增率分别为0.0%?25.4%和80.0%,差异有统计学意义(P < 0.01);CD105标记的微血管密度(MVD)亦存在明显差异(P < 0.01)?宫颈CA中,hTERC基因扩增率和MVD呈显著正相关(P < 0.05),均随CIN级别的升高而增加,其中CA-CIN Ⅰ级与CA-CIN Ⅱ级之间差异有统计学意义(P < 0.01);两者表达在复发组均显著高于未复发组(P < 0.05)?结论:hTERC基因扩增和CD105表达在宫颈CA复发并向高级别CIN转化过程中扮演着重要角色,对宫颈CA患者检测hTERC基因扩增和CD105表达有助于临床筛查高危CA患者?
英文摘要:
      Objective:To evaluate the significance of hTERC gene amplification and CD105 expression in cervical condyloma acuminateum (CA). Methods:Fluorescence in situ hybridization (FISH)and imunohistochemical staining were used to detect the expression of hTERC gene and CD105 in 67 cases of cervical CA,including 31 cases without CIN,19 CA-CIN grade Ⅰ,11 CA-CIN grade Ⅱ,and 6 CA-CIN grade Ⅲ; 35 cases of cervical cancer and 24 normal cervical mucosa were performed as controls. Results:The rates of hTERC gene amplification were 0%,25.4% and 80% in normal cervical mucosa,cervical CA and cervical cancer,respectively(P < 0.01). Microvessel density(MVD) marked by CD105 antibody also had significant difference (P < 0.01). In cervical CA,hTERC gene amplification and MVD showed a positive correlation (P < 0.05)and increased with increasing CIN grade. There were significant differences between CA-CIN Ⅰ and CA-CIN Ⅱ(P < 0.01),as well as between the recurrence group and the non recurrence group(P < 0.05). Conclusion:The expression of hTERC gene and CD105 may play an important role in the recurrence of cervical CA,and in the process of conversion to the high grade CIN. The detection of hTERC gene and CD105 is helpful in the clinical screening of high-risk patients with cervical CA.
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