文章摘要
丁思阳,陈 宁,沈 铭.纯钛表面二氧化钛纳米管对人牙周膜干细胞增殖及成骨分化的影响[J].南京医科大学学报,2016,(12):1426~1430
纯钛表面二氧化钛纳米管对人牙周膜干细胞增殖及成骨分化的影响
Effect of titanium modified by anodic oxidized TiO2 nanotubes on human periodontal ligament stem cells
投稿时间:2016-06-27  
DOI:10.7655/NYDXBNS20161204
中文关键词: 纳米管  钛材  人牙周膜干细胞  成骨分化
英文关键词: nanotubes  pure titanium  hPDLSCs  osteogenic differentiation
基金项目:
作者单位
丁思阳 南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院种植修复科,江苏 南京 210029 
陈 宁 南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院种植修复科,江苏 南京 210029 
沈 铭 南京医科大学口腔疾病研究江苏省重点实验室,南京医科大学附属口腔医院种植修复科,江苏 南京 210029 
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中文摘要:
      目的:探讨纯钛表面二氧化钛(TiO2)纳米管改性后对人牙周膜干细胞(human periodontal ligament stem cells,hPDLSCs)增殖?成骨分化能力的影响?评价TiO2纳米管改性作为种植体表面改性方法的作用?方法:将实验组钛片进行阳极氧化TiO2纳米管处理,对照组仅进行抛光处理?将hPDLSCs与两组钛材分别复合培养,检测其增殖及成骨相关蛋白的表达水平?结果:成功在钛材表面制备了多孔?有序?管径约为100 nm的TiO2纳米管;实验组hPDLSCs较对照组1 d的增殖活性及4 d的ALP活性没有明显差异;而4?7 d实验组细胞的增殖活性低于对照组;而7 d?14 d及21 d实验组细胞ALP活性高于对照组;21 d时,实验组 Col-I?OCN?Runx-2的基因表达水平明显高于对照组?结论:通过阳极氧化可在钛材表面制备多孔有序的TiO2纳米管层;且TiO2纳米管能有效促进hPDLSCs在钛表面的骨向分化?
英文摘要:
      Objective:To investigate the effect of titanium modified by anodic oxidized TiO2 nanotubes on the osteogenic differentiation of human periodontal ligament stem cells(hPDLSCs). Methods:TiO2 nanotubes group and control group(smooth titanium)were involved in this study. The cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay. The osteogenic differentiation was evaluated using alkaline phosphatase(ALP)activity assay. In addition,osteogenic marker genes expression including Col-I,OCN and Runx-2 was evaluated. Results:We prepared nominally TiO2 nanotubes with 100 nm diameter on Ti substrates by anodization. No significant difference was detected on the proliferative activity at day 1 and the ALP activity at day 4 between the TiO2 nanotubes group and control group(P > 0.05). However,cell proliferative activity of TiO2 nanotubes group was lower than that of the control group at day 4 and 7. ALP activity assay demonstrated that the TiO2 nanotubes group at day 7,14 and 21 had a high ALP activity. The gene expression of Col-1,OCN,and Runx-2 of TiO2 nanotubes group was up-regulated at 21-days. Conclusion:The proliferation activity of hPDLSCs on the TiO2 nanotubes was less than that of control group. ALP activity,gene expression TiO2 nanotubes group were higher than those of on control group,and it suggested that TiO2 nanotubes could promote osteogenic differentiation of hPDLSCs.
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