文章摘要
陈惠新,欧志英,陈丽莎,徐永成,曾书君,罗 程,余志金.IL-8受体介导c-Jun和Ets-1异常调控MMP-9促进胃癌细胞侵袭[J].南京医科大学学报,2017,(2):184~188
IL-8受体介导c-Jun和Ets-1异常调控MMP-9促进胃癌细胞侵袭
IL-8 receptor mediated c-Jun and Ets-1 abnormal promoted gastric cancer cells metastasis through MMP-9
投稿时间:2016-02-13  
DOI:10.7655/NYDXBNS20170210
中文关键词: 胃癌  侵袭  IL-8受体  c-Jun  Ets-1  MMP-9
英文关键词: Gastric cancer  Metastasis  IL-8 receptor  c-Jun  Ets-1  MMP-9
基金项目:广东省自然科学基金面上项目(S201310011923);惠州市科技计划项目(2013Y009)
作者单位
陈惠新 惠州市中心人民医院消化内科,广东 惠州 516001 
欧志英 广州市妇女儿童医疗中心,广东 广州 510623 
陈丽莎 惠州市中心人民医院消化内科,广东 惠州 516001 
徐永成 惠州市中心人民医院消化内科,广东 惠州 516001 
曾书君 惠州市中心人民医院消化内科,广东 惠州 516001 
罗 程 惠州市中心人民医院消化内科,广东 惠州 516001 
余志金 惠州市中心人民医院消化内科,广东 惠州 516001 
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中文摘要:
      目的:探讨白介素-8(IL-8)受体促进胃癌侵袭所介导的分子机制。方法:胃癌细胞培养后分别转染空白质粒pcDNA3.1(+)?针对IL-8受体的反义表达质粒pcDNA3.1(+)/as-IL-8G和针对IL-8受体的正义表达质粒pcDNA3.1(+)/s-IL-8G,Western blot检测蛋白表达,细胞侵袭力用相对侵袭细胞数量表示。结果:低表达IL-8受体的胃癌细胞侵袭能力显著减弱,且细胞内c-Jun?Ets-1?MMP-9的蛋白水平显著下降;而高表达IL-8受体的胃癌细胞侵袭能力则显著增强,且细胞内c-Jun?Ets-1?MMP-9的蛋白水平显著上升。用硫代磷酸化修饰的反义寡聚脱氧核苷酸阻断该细胞内c-Jun或Ets-1的表达后,MMP-9的蛋白表达水平显著降低,并伴随细胞侵袭能力明显下降。结论:IL-8受体通过介导c-Jun和Ets-1异常调控MMP-9促进了胃癌细胞的侵袭过程。
英文摘要:
      Objective:To explore the molecular mechanism in which IL-8 receptor promoted gastric cancer metastasis. Methods:Gastric cancer cells were cultured and transfected with IL-8 receptor antisense expression plasmid pcDNA3.1(+)/as-IL-8G and IL-8 receptor sense expression plasmid pcDNA3.1(+)/s-IL-8G. Protein expression was detected by Western blotting. Cell invasion was performed in Transwell and the invasion capacity was expressed by the relative invasive cell number. Results:Gastric cancer cells with lower expression of IL-8 receptor had significantly reduced invasion ability,and the expression of c-Jun,Ets-1 and MMP-9 decreased correspondingly,while higher expression of IL-8 receptor markedly enhanced the invasion capacity of gastric cancer cells,and the expression of c-Jun,Ets-1 and MMP-9 increased significantly. MMP-9 protein expression level decreased significantly after blocking the c-Jun or Ets-1 expression by phosphorylated modification antisense oligonucleotide technology,and the cell invasion ability decreased obviously at the same time. Conclusion:IL-8 receptor promoted gastric cancer cells metastasis through abnormal c-Jun and Ets-1-mediated MMP-9.
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