文章摘要
姜黎黎,魏晨晨,陈沁楠,陈臻瑶,陈 昕,王朝霞.长非编码RNA BANCR逆转非小细胞肺癌细胞株顺铂耐药的研究[J].南京医科大学学报,2017,(2):189~193
长非编码RNA BANCR逆转非小细胞肺癌细胞株顺铂耐药的研究
BRAF activated non-coding RNA (BANCR)reverse human non-small cell lung cancer A549/DDP cell line to cisplatin resistance
投稿时间:2016-03-19  
DOI:10.7655/NYDXBNS20170211
中文关键词: 非小细胞肺癌  BANCR  顺铂耐药性
英文关键词: NSCLC  BANCR  DDP-resistance
基金项目:国家自然科学基金(81272601,81472198);江苏省科技厅临床医学科技专项(BL2014096);江苏省医学重点人才基金(RC2011080)
作者单位
姜黎黎 南京医科大学第二附属医院肿瘤科,江苏 南京 210011
海门市人民医院肿瘤科,江苏 海门 226100 
魏晨晨 南京医科大学第二附属医院肿瘤科,江苏 南京 210011 
陈沁楠 南京医科大学第二附属医院肿瘤科,江苏 南京 210011 
陈臻瑶 南京医科大学第二附属医院肿瘤科,江苏 南京 210011 
陈 昕 南京医科大学第二附属医院肿瘤科,江苏 南京 210011 
王朝霞 南京医科大学第二附属医院肿瘤科,江苏 南京 210011 
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中文摘要:
      目的:研究长非编码RNA BANCR对人非小细胞肺癌耐顺铂细胞株A549/DDP耐药性的影响及其可能的作用机制。方法:应用实时荧光定量PCR法(qRT-PCR)检测耐顺铂细胞株A549/DDP及其亲本细胞株A549中BANCR的表达差异,发现BANCR在A549/DDP细胞中显著低表达。在A549/DDP细胞中过表达BANCR,分别应用MTT法?克隆形成实验?流式细胞术检测过表达后A549/DDP细胞对顺铂药物敏感性,细胞增殖能力,联合顺铂处理后细胞凋亡变化;Western blot检测过表达BANCR后A549/DDP细胞p53的表达变化。结果:BANCR在A549/DDP细胞中的表达量显著低于A549细胞(P < 0.05),在A549/DDP细胞中过表达BANCR可产生以下效应:相较于对照组,顺铂对A549/DDP/BANCR细胞的半数抑制浓度(half inhibition concentration,IC50)减低(P < 0.05),A549/DDP/BANCR细胞的增殖能力减弱,A549/DDP/BANCR经过顺铂处理后细胞凋亡增多(P < 0.05)。Western blot结果显示,与空白对照组比较,过表达BANCR的 A549/DDP细胞p53表达水平明显升高。结论:BANCR可能通过诱导细胞凋亡,抑制细胞增殖,上调p53蛋白表达而逆转 A549/DDP细胞对DDP的耐药性。
英文摘要:
      Objective:To explore the effect of BANCR in reversing cisplatin(DDP) resistance of human non-small cell lung cancer(NSCLC)A549/DDP cell line and its related mechanisms. Methods:The real-time fluorescent quantitative PCR(qRT-PCR) was used to detect BANCR expression differences in DDP-resistant A549/DDP cells and its parent cell line A549.Overexpress BANCR in A549/DDP cells and use MTT assay,colony formation assay and flow-cytometric analysis to detect A549/DDP cells to DDP drug sensitivity,cell proliferation ability and apoptosis on treatment with DDP.The protein expression of p53 in A549/DDP cells were detected by western blotting. Results:The BANCR expression level in A549/DDP cell line was significantly lower than that in A549 cell line (P < 0.05). Ectopic BANCR expression in A549/DDP cells could produce the following effects:compared to the control group,the half inhibition concentration (IC50) of DDP in A549/DDP/BANCR cells lower (P < 0.05),proliferation of A549/DDP/BANCR cells diminished(P < 0.05),apoptosis on treatment with DDP increased(P < 0.05). Furthermore,the protein expressions p53 was upregulated in BANCR transfected group than those in empty vector. Conclusion:BANCR reverses the resistance of A549/DDP cells to DDP through induction of apoptosis,diminished cell proliferation,up-regulating the expressions of p53 protein.
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