Objective:To survey the prevalence of drug resistant and subtypes HIV in Jiangsu province in 2015. Methods:WHO truncated sequential sampling technique was adopted by using 47 samples of newly diagnosed as HIV positive and aged <25 years in Nanjing. RNA was extracted and HIV pol region was amplified by RT-PCR and nested PCR. Pol genetic mutation associated with transmitted drug resistance(TDR)was analyzed. Results:The success rate for sequence acquisition in the survey was 100%(47/47). The main subtype was CRF01_AE(59.6%),the rest subtypes in the sequence were CRF07_BC(25.5%),C(6.4%),B(2.1%),additional with 3 URFs. The prevalence of TDR in Jiangsu was low,and only 1 surveillance drug-resistance mutation(SDRMs)in protease inhibitor(PI)(M46I)was found by analyzing the 47 sequences. The individual with drug resistant HIV stain found in this study was infected by homosexual transmission and the subtype was CRF01_AE. The point finding in this study is that the percentage of HIV students was 46.8%(22/47),exceeding the percentage of HIV business servicers of 29.8%(14/47). A total of individuals with HIV were infected by homosexual transmission,except one by heterosexual. Conclusion:TDR has arisen in Jiangsu. Though the prevalence was low,we should still pay a closed attention on the tendency of TDR change. Students under age 25 are becoming the main constitution in newly diagnose HIV infections. Scanning up on consulting,testing and behavioral intervention are beneficial to HIV prevention and control. The key point on prevalence of TDR is to take preventive measures between men who have sex with men(MSMs).