文章摘要
吴奕章,韦震鸣,吴英智,王慧敏,傅 强,李志樑.真武汤对2型心肾综合征模型大鼠心脏和肾脏的保护作用[J].南京医科大学学报,2017,(5):526~531,553
真武汤对2型心肾综合征模型大鼠心脏和肾脏的保护作用
Protective effect of Zhenwu Decoction on heart and renal tissue of Type 2 CRS model rats
投稿时间:2016-12-13  
DOI:10.7655/NYDXBNS20170502
中文关键词: 真武汤  心肾综合征  纤维化  硫酸吲哚酚
英文关键词: Zhenwu Decoction  cardiorenal syndrome  fibrosis  indoxyl-sulfate
基金项目:国家自然科学基金面上项目(81573732);广东省自然科学基金项目(2015A030313304)
作者单位
吴奕章 南方医科大学珠江医院心血管内科广东 广州 510282 
韦震鸣 南方医科大学珠江医院心血管内科广东 广州 510282 
吴英智 南方医科大学珠江医院心血管内科广东 广州 510282 
王慧敏 南方医科大学珠江医院心血管内科广东 广州 510282 
傅 强 南方医科大学珠江医院心血管内科广东 广州 510282 
李志樑 南方医科大学珠江医院心血管内科广东 广州 510282 
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中文摘要:
      目的: 探讨真武汤对2型心肾综合征大鼠心脏和肾脏的保护作用。方法: 通过结扎左冠状动脉前降支联合肾大部分切除手术构建2型心肾综合征大鼠模型。32只SD大鼠随机分成真武汤低、中、高浓度干预组和对照组。干预组分别给予不同生药量的真武汤灌胃,对照组给予等剂量蒸馏水灌胃。干预结束后比较大鼠的生化指标、心脏超声检测和免疫组化结果。结果: 高浓度组血尿素氮、血肌酐较对照组明显降低,尿硫酸吲哚酚较对照组明显升高,且高浓度组血尿素氮较低浓度组明显降低(P<0.05)。中浓度组血尿素氮、血肌酐较对照组明显降低(P<0.05)。低、中、高浓度组左室后壁(收缩期)、心排量、每搏输出量较对照组明显增加(P<0.05)。另外高浓度组较对照组左室内径(舒张)和左室容积(收缩)明显增加(P<0.05)。Masson染色、CollagenⅠ免疫荧光染色、TGF-β 免疫荧光染色均表明低、中、高浓度组的心脏和肾脏组织纤维化较对照组减轻。结论: 真武汤对2型心肾综合征大鼠心脏和肾脏具有一定的保护作用。该作用可能通过增加尿毒素排出和减轻组织纤维化来实现。
英文摘要:
      Objective: To investigate the Protective effect of Zhenwu Decoction on heart and renal tissues of type 2 CRS model rats. Methods: We built type 2 CRS model rats by combining ligation of left descending coronary artery and subtotal nephrectomy. Thirty-two rats were randomly divided into low, middle, high dose treatment and control groups. Treatment groups were given different concentration but same volume decoction for lavage and control group was treated with same amount of distilled water. Serum index, echocardiogram and immunohistochemical results were compared. Results: Compared with the control group, the high dose treatment group has reduced serum Cr and BUN level but higher excretion of urine indoxyl-sulfate (P<0.05). Serum Cr and BUN levels were lower in middle dose treatment group than the control group (P<0.05). All treatment groups had a higher amount of Lvpw(systolic),CO and SV than the control group. Addition,the high dose treatment group has increased diameter and volume of left ventricle. Masson staining, Collagen I and TGF-β immunofluorescensce results all demonstrate less fibrosis in heart and kidney tissues of the treatment groups than the control group. Conclusion: Zhenwu Decoction has protective effects on heart and renal tissue of Type 2 CRS model rats. This effect may be achieved by increase uremic toxin excretion and ameliorating fibrosis.
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