文章摘要
童佩,奚玲,杨洁,刘云.S100A16基因敲除小鼠模型的构建与鉴定[J].南京医科大学学报,2017,(5):549~553
S100A16基因敲除小鼠模型的构建与鉴定
Establishment and identification of S100A16 gene knockout mouse model
投稿时间:2017-01-07  
DOI:10.7655/NYDXBNS20170506
中文关键词: S100A16  基因敲除  动物模型
英文关键词: S100A16  gene knockout  animal model
基金项目:国家自然科学基金(81270952)
作者单位
童佩 南京医科大学第一附属医院老年医学内分泌科江苏 南京 210029 
奚玲 南京医科大学第一附属医院老年医学内分泌科江苏 南京 210029 
杨洁 南京医科大学第一附属医院老年医学内分泌科江苏 南京 210029 
刘云 南京医科大学第一附属医院老年医学内分泌科江苏 南京 210029 
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中文摘要:
      目的:S100A16在肥胖以及多种恶性肿瘤发生发展中发挥了重要作用,本研究拟构建S100A16基因敲除小鼠模型。方法:利用基因表达调控系统(即Cre/lox P系统)构建全身敲除小鼠。采用聚合酶链式反应(PCR)鉴定小鼠的基因型,采用实时定量PCR(QRT-PCR)、Western blot方法验证其转录及翻译水平的表达。结果:成功建立了S100A16全身敲除小鼠模型,基因敲除杂合子小鼠成功饲养繁殖,目前为止未出现纯合子小鼠。S100A16敲除小鼠主要代谢器官,如脂肪、肌肉、肝脏中S100A16蛋白表达量显著下降。结论:S100A16 基因敲除小鼠模型的构建为研究肥胖及胰岛素抵抗中的作用及机制提供了动物模型。
英文摘要:
      Objective:To establish the S100A16 gene knockout mouse model,which can be used for the study on its biologic function. Methods:To establish the S100A16 gene knockout mouse model via Cre/loxP system. PCR was used to identify the genotype of the offspring,the expression level of S100A16 mRNA was detected by qRT-PCR,and expression of S100A16 protein was detected by Western blot. Results:S100A16 gene knockout mouse model has been successfully established. Heterozygous mice were successfully bred and reproduced. So far,gene knockout homozygous mice were not found. Conclusion:The S100A16 mouse could be a useful model for the researches on its function,especially in obesity and insulin resistance.
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