文章摘要
张 璐,李晓璐,李宛桐,徐 莹,刘鹏利.Gal-1,ANXA2 和 PRPH 在SD大鼠耳蜗核的表达[J].南京医科大学学报,2017,(5):575~579
Gal-1,ANXA2 和 PRPH 在SD大鼠耳蜗核的表达
Expression of Gal-1,ANXA2 and PRPH in cochlear nucleus on SD rats
投稿时间:2017-01-27  
DOI:10.7655/NYDXBNS20170511
中文关键词: 中枢听觉处理障碍  CN  Gal-1  ANXA2  PRPH
英文关键词: CAPD  CN  Gal-1  ANXA2  PRPH
基金项目:江苏省“科教兴卫工程”医学重点人才(RC2011073)
作者单位
张 璐 南京医科大学第一附属医院耳鼻咽喉科WHO预防聋和听力减退合作中心江苏 南京 210029 
李晓璐 南京医科大学第一附属医院耳鼻咽喉科WHO预防聋和听力减退合作中心江苏 南京 210029 
李宛桐 南京医科大学第一附属医院耳鼻咽喉科WHO预防聋和听力减退合作中心江苏 南京 210029 
徐 莹 南京医科大学第一附属医院耳鼻咽喉科WHO预防聋和听力减退合作中心江苏 南京 210029 
刘鹏利 南京医科大学第一附属医院耳鼻咽喉科WHO预防聋和听力减退合作中心江苏 南京 210029 
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中文摘要:
      目的:采用同位素相对标记和绝对定量(isobaric tags for relative and absolute quantitation,iTRAQ)技术分析耳蜗核(cochlear nucleus,CN)区域特异性膜蛋白。方法:出生60 d的雄性SD大鼠分为4组,分别是CN、下丘(inferior colliculus,IC)、上橄榄核(superior oliver complex,SOC)、大脑其余部位(Rest)。提取这4个部位组织的细胞膜蛋白,再采用iTRAQ技术检测出CN区域特异性蛋白。通过Uniprot检索这些蛋白的功能和参与的生物代谢过程。结果:本实验最终得到17种CN区域特异性膜蛋白。对CN区域蛋白进行基因本体(gene ontology,GO)分析发现,这些蛋白主要参与生物调节与发育。UNIPROT检索发现3种蛋白与神经系统功能相关,即半乳糖凝集素-1(galectin-1,Gal-1)、膜联蛋白A2(annexin-A2,AXNA2)和外周蛋白(peripherin,PRPH)。Gal-1与神经生长发育相关;AXNA2参与多种生物调节过程;PRPH与神经退行性病变相关。其中,Gal-1可促进轴突生长、调节突触可塑性且具有神经保护作用;AXNA2可能参与了神经递质释放过程,且有神经营养作用;PRPH在高表达时具有神经毒性,和神经丝蛋白共同参与神经退行性病变。结论:Gal-1、ANXA2 和PRPH在耳蜗核中高度表达,可能与中枢信息处理相关,这为进一步研究中枢听觉处理障碍发病机制提供了一定的理论基础。
英文摘要:
      Objective:Using isobaric tags for relative and absolute quantitation(iTRAQ),to find the region-typical membrane proteins of cochlear nuclues(CN).Methods:The subjects are male D60 SD rats.The four regions CN,Inferior Colliculus(IC) ,Superior Oliver Com-plex(SOC) and the rest of the brain(Rest) ,were seperated. The region-typical proteins of CN were identified with iTRAQ. The functions of these proteins and the process of biological metabolism involved were discussed seriously through Uniprot.Results:Seventeen region-typical proteins of CN were identified.GO(Gene Ontology) pathway analysis indicates that they are mainly involved in biological regulation processes and development. Gal-1(Galectin-1),ANXA2(Annexin-A2), and PRPH(Peripherin)were found to be related to the function of the nervous system through Uniprot.Gal-1 was involved in neural development and synapase plasticity,and has neuroprotective effects. ANXA2 had neurotrophic effects, and might be involved in the release of neurotransmitters. Highly expressed PRPH led to neurotoxi-city, and was involed in neurodegenerative disease.Conclusion: In CN, Gal-1, ANXA2 and PRPH proteins might be related to central audi-tory processing, and it provides theoretical basis for further researches on the pathogenesis of central auditory processing disorder.
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