文章摘要
张 吟,许小凤.NF-κ B和MMP-9在亚临床甲减孕鼠胎盘中的表达及意义[J].南京医科大学学报,2017,(5):580~583
NF-κ B和MMP-9在亚临床甲减孕鼠胎盘中的表达及意义
Expression and significance of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism
投稿时间:2017-02-17  
DOI:10.7655/NYDXBNS20170512
中文关键词: 亚临床甲减  胎盘  核转录因子-κB 基质金属蛋白酶-9  免疫组织化学法  Western blot
英文关键词: subclinical hypothyroidism  placenta  Nuclear Kappa B  matrix metalloproteinase-9  immunohistochemistry  Western blot
基金项目:苏州市科技局科技发展计划(SYSD2012115,SYSD2013126);苏州市卫生局科技项目(LCZX201512)
作者单位
张 吟 南京中医药大学附属常州市中医医院妇科江苏 常州 213003 
许小凤 南京中医药大学附属苏州市中医医院生殖医学科江苏 苏州 215000 
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中文摘要:
      目的:通过研究核转录因子-κB (Nuclear Kappa B,NF-κB)和基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)在亚临床甲减孕鼠胎盘组织中的变化,分析亚临床甲减孕鼠早期先兆流产的发生机制。方法:采用免疫组织化学法、Western blot检测NF-κB和MMP-9在亚临床甲减孕鼠胎盘组织中(E10)和正常组中的表达情况,分析二者与亚临床甲减孕鼠早期先兆流产的相关性。结果:NF-κB和MMP-9在亚临床甲减孕鼠胎盘组织中的阳性表达率分别为16.7%和50.0%,而在正常组胎盘组织中的阳性率分别为83.3%和100.0%,差异均有统计学意义;Western blot检测结果与免疫组织化学检测结果基本一致。结论:NF-κB和MMP-9在亚临床甲减孕鼠胎盘组织中低表达,导致滋养细胞侵袭力下降,对判断妊娠期亚临床甲减早期先兆流产发生机制具有重要指导意义。
英文摘要:
      Objective: To explore the expressions of Nuclear Kappa B (NF-κB) and matrix metalloproteinase-9 (MMP-9) in placental tissues of pregnant rats with subclinical hypothyroidism and study the occurrence mechanism of threatened abortion. Methods: The expressions of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism and normal pregnant rats were detected by immunohistochemistry and Western blotting assay. Correlations between the expressions and the occurrence mechanism of threatened abortion of subclinical hypothyroidism were analyzed. Results: The positive rates of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism were 16.7% and 50% ,respectively,while in normal placental tissues were 83.3% and 100% ,respectively.The differences were significant(all P<0.05).The results of Western blotting assay were consistent with those of immunohistochemistry. Conclusion: The expression levels of NF-κB and MMP-9 in placental tissues of pregnant rats with subclinical hypothyroidism have a high correlation with the invasion,and joint detection of the above indicators ,and it is instructional for judgement the occurrence mechanism of threatened abortion of subclinical hypothyroidism.
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