文章摘要
王立文,沈晓洁,何文涓,吴 倩,嵇莹莹,龚国清.NF-κB水平在大鼠血液高凝态下的变化[J].南京医科大学学报,2017,(8):990~993
NF-κB水平在大鼠血液高凝态下的变化
Changes of NF-κB in rat blood hypercoagulable state
投稿时间:2016-07-19  
DOI:10.7655/NYDXBNS20170814
中文关键词: 高凝态  NF-κB  大鼠
英文关键词: hypercoagulable state  level of NF-κB  rats
基金项目:无锡市医院管理中心科研项目(YGZXM1533)
作者单位
王立文 无锡卫生高等职业技术学校护理系江苏 无锡 214028 
沈晓洁 无锡卫生高等职业技术学校护理系江苏 无锡 214028 
何文涓 无锡卫生高等职业技术学校护理系江苏 无锡 214028 
吴 倩 中国药科大学药学院药理系江苏 南京 210009 
嵇莹莹 中国药科大学药学院药理系江苏 南京 210009 
龚国清 中国药科大学药学院药理系江苏 南京 210009 
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中文摘要:
      目的:观察核因子κB(NF-κB)水平在大鼠血液高凝态下的变化,为血栓疾病的防治探索新靶点。方法:大鼠80只随机分为模型组与生理盐水组,模型组以预实验确定的最适剂量盐酸肾上腺素造模,于12、18、24、48、96 h各组随机取8只大鼠颈动脉取血测凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、全血黏度,并采用酶联免疫吸附法测NF-κB水平。结果:0.7 mg/kg肾上腺素造模组模型复制成功率高,大鼠死亡率低,为最佳造模剂量。生理盐水组PT、APTT、全血黏度及NF-κB水平在各时间点无统计学差异;模型组PT、APTT较生理盐水组缩短(P<0.05),但随时间缓慢延长,全血黏度及NF-κB水平较生理盐水组增大(P<0.05),但随时间缓慢降低,且在12、18 h时PT、APTT同生理盐水组比较有显著差异(P<0.05),24 h内全血黏度较生理盐水组显著增大(P<0.05),48 h内NF-κB水平较生理盐水组显著升高(P<0.05)。结论:高凝态大鼠血液中NF-κB水平显著升高,并随着高凝态的消退而降低,说明大鼠血液高凝态的发生、发展可能与NF-κB信号通路表达上调有关。
英文摘要:
      Objective:To verify changes of NF-κB in hypercoagulable state of rats and find new targets to treat thrombotic diseases. Methods:Eighty rats were splited into model group and control group. The model group was injected with adrenaline hydrochloride at the dosage determined in pre-experiment,while the control group was injected with normal saline. After 12,18,24,48 and 96 h,eight rats of each group were anaesthetized to collect blood sample. Preparing plasma samples were prepared to test PT,APTT, and the whole blood viscosity, and serum were collected to test the level of NF-κB. Results:In the pre-experiment,death rate of the model group of 0.7 mg/kg was lower,and compared with the control group,PT and APTT were shorter(P<0.05) and the whole blood viscosity was higher(P<0.05);The model group showed distinct differences on PT and APTT compared with the control group at time of 12 h and 18 h(P<0.05),the whole blood viscosity of the model group is higher than that of the control group within 24 h,and the level of NF-κB of the model group is higher than that of the control group within 48 h. Conclusion:The best dosage of adrenaline is 0.7 mg/kg. Level of NF-κB in rats with hypercoagulable state is higher than that of the control group,which decreases with time. The hypercoagulable state in rats may be related to the increasing expression of NF-κB pathway.
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