多光子显微成像技术用于诊断结直肠疾病可行性的初步研究
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国家自然科学基金(81700577,81302107);江苏省科技临床专项(BL2012031);江苏省“333”工程基金(BRA2015472);江苏省青年医学人才项目(QNRC2016569)


Initial investigation of the feasibility of real⁃time multiphoton microscopic imaging for diagnosing colorectal diseases
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    目的:通过多光子显微成像技术(multiphoton microscopic imaging,MPM)观察并初步建立结直肠组织的镜下特点,探讨MPM用于肠道疾病的诊断价值。方法:回顾性分析100个经病理证实为正常、增生性息肉、腺瘤、腺癌的离体新鲜组织的MPM图像,初步总结 MPM诊断结直肠疾病的特点。结果:通过观察比较各类型的MPM图片,发现从隐窝开口、腺体结构、腺上皮细胞及胶原纤维可对结直肠疾病进行MPM诊断。正常、增生性、低级别上皮内瘤变、高级别上皮内瘤变和癌变组织的细胞核面积平均值各为(32.14 ± 1.96)μm2、(32.69 ± 2.34)μm2、(55.40 ± 4.63)μm2、(71.83 ± 5.30)μm2、(85.90 ± 11.50)μm2,正常上皮细胞与增生性上皮细胞的细胞核面积无统计学差异(P=0.517),其余各两组细胞核面积之间均有统计学差异(P< 0.001)。结论:多光子显微成像技术可以无损、实时、高分辨率地对结直肠疾病进行分类及分级诊断,未来有望与内镜整合,成为新一代光学诊断技术。

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    Objective:To investigate and establish the microscopic features of colorectal diseases via multiphoton microscopy(multiphoton microscopic imaging,MPM)initially,evaluating the diagnostic value in vitro. Methods:The multiphoton images of 100 fresh tissues confirmed pathologically colorectal normal tissues,hyperplastic polyps,adenomas and adenocarcinomas were collected and retrospectively analyzed to establish diagnostic standards with MPM. Results:Through the investigation,we found the colorectal normal,hyperplastic polyps,adenoma and adenocarcinoma were different in the crypt opening,glandular structure,epithelial cells and collagen fibers. Besides,the cellular areas by quantitive analysis were 32.14 ± 1.96 μm2、32.69 ± 2.34 μm2、55.40 ± 4.63 μm2、71.83 ± 5.30 μm2、85.90 ± 11.50 μm2,respectively. P values were both of statistically significance. Conclusion:The real-time multiphoton microscopic imaging can be used to classify and grade the colorectal diseases safely and effectively with high resolution. It is potential to become the new generation of optical technique via integrating with the endoscopes in the future.

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何可心,赵黎黎,黄晓阳,丁 颖,化宏金,刘 莉,王 翔,王 敏,张 勇,范志宁.多光子显微成像技术用于诊断结直肠疾病可行性的初步研究[J].南京医科大学学报(自然科学版),2018,(2):201-205,238

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  • 收稿日期:2017-10-17
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  • 在线发布日期: 2018-03-02
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