文章摘要
费 毫,翟晨骏,王 振,张 啸,范卫民.以改良方法制作软骨细胞外基质构建高质量组织工程软骨[J].南京医科大学学报,2018,(7):934~939,987
以改良方法制作软骨细胞外基质构建高质量组织工程软骨
Utilizing cartilage extracellular matrix⁃derived scaffolds prepared by a modified method for constructing tissue engineered cartilage
投稿时间:2018-04-03  
DOI:10.7655/NYDXBNS20180712
中文关键词: 组织工程  软骨细胞外基质  细胞⁃支架复合物
英文关键词: tissue engineering  cartilage extra matrix  cell⁃scaffold construct
基金项目:国家自然科学基金(81472079,81672169)
作者单位
费 毫 南京医科大学第一附属医院骨科江苏 南京 210029 
翟晨骏 南京医科大学第一附属医院骨科江苏 南京 210029 
王 振 南京医科大学第一附属医院骨科江苏 南京 210029 
张 啸 南京医科大学第一附属医院骨科江苏 南京 210029 
范卫民 南京医科大学第一附属医院骨科江苏 南京 210029 
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中文摘要:
      目的:探讨应用改良方法制作的软骨细胞外基质(extracellular matrix,ECM)能否构建高质量组织工程软骨。方法:应用传统匀浆?脱细胞方法制作软骨ECM粉末,在此基础上通过物理研磨颗粒筛选获得细腻的软骨细胞外基质粉末(fine extracellular matrix,FECM)。将两种粉末通过冷冻干燥法制成ECM支架和FECM支架作为对照组和实验组。以软骨组织工程中成熟应用的聚乳酸聚羟基乙酸聚合物[poly(lactic?co?glycolic acid),PLGA]支架为阳性对照组。取等量大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)种植于ECM支架和FECM支架,同时将BMSCs诱导分化为软骨细胞后种植于PLGA支架,然后将3种细胞?支架复合物移植入裸鼠皮下,28 d后取出样本并进行相关检测。结果:FECM支架孔径均一,结构规整,其构建的组织工程软骨在大小、色泽、糖胺多糖(sGAG)分泌以及胶原沉积等方面均远优于对照组构建的组织工程软骨,与阳性对照组无明显差异。结论:由改良方法制作的FECM支架在无外源性转化生长因子?β1(transforming growth factor?β1,TGF?β1)情况下可构建出高质量的组织工程软骨,具有潜在的临床应用价值。
英文摘要:
      Objective:To evaluate the capacity of cartilage extracellular matrix?derived scaffolds prepared by a modified method for constructing tissue engineered cartilage. Methods:The cartilage pieces were fragmented using two different methods to produce extracellular matrix(ECM) powder and fine extracellular matrix(FECM) powder. ECM scaffold and FECM scaffold were fabricated with ECM powder and FECM powder through freeing and lyophilization technique. Both of ECM and FECM scaffolds were seeded with bone marrow mesenchymal stem cells(BMSCs)as the control group and the experimental group. The poly(lactic?co?glycolic acid)(PLGA) scaffold were seeded with BMSCs induced to differentiate into chondrocytes as the positive control group. After cultured in vitro for 12 h,the cell?scaffold constructs were implanted in the nude mice subcutaneously. The cell?scaffold constructs were harvested and detected on histology and immunohistochemistry at day 28. Results:Cell?scaffold constructs of the experimental group grew much better than that of the control group,and similar to the postive control group. More cartilage?like tissues were generated using FECM scaffold and stained strongly for sGAGs and type Ⅱ collagen. Conclusion:FECM scaffold can promote the differentiation of BMSCs into chondrocytes without exogenous transforming growth factor?β1(TGF?β1) and have potential of application in tissue engineering cartilage .
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