Objective：To screen B cell and T cell antigen epitopes of Acinetobacter baumannii protein TonB. Methods：B cell epitopes and T cell epitopes were predicted by bioinformatics methods and synthesized. Recombinant pET28a-tonB was cloned and TonB was purified to immunize mice. BALB/c mice were immunized by subcutaneous for 3 times. Five weeks later，serum was collected from each of immunized mice and then negative control were mice used to perform indirect ELISA with synthesized peptides to identify B cell epitopes. Cells of the spleen were separated from every mouse and cultured with each of synthesized peptides respectively，and then cell supernate was collected to detect IFN-γ secretion by double sandwich ELISA for T cell epitopes selection. Results：Construction of 4 candidate B cell and T cell epitopes was performed，including PB1，PB2，PB3，PB4，PT1，PT2，PT3，and PT4，respectively. Indirect ELISA showed that peptide PB3，PB4 reacted with immunized mice serum and the A450 nm value was significant higher than that of the negative mice（P＜0.05）. Double sandwich ELISA showed that IFN-γ concentration that stimulated by peptide PT2，PT3，and PT4 was significant higher than its negative control group（P＜0.05）. Conclusion：Two B cell epitopes PB3 and PB4，3 T cell epitopes PT2，PT3，PT4 of TonB were successfully constructed and screened.