文章摘要
史 莹,毛 山,叶 亮,谷 伟.Kv1.3通道在哮喘患者外周血T淋巴细胞中的表达及对Th2细胞因子的影响[J].南京医科大学学报,2018,(10):1361~1364
Kv1.3通道在哮喘患者外周血T淋巴细胞中的表达及对Th2细胞因子的影响
Expression and function of Kv1.3 channel in peripheral blood T lymphocytes of asthma patients
投稿时间:2018-03-15  
DOI:10.7655/NYDXBNS20181006
中文关键词: 哮喘  Kv1.3钾通  T淋巴细胞
英文关键词: asthma  Kv1.3 channel  T lymphocyte
基金项目:南京市医学科技发展项目(JQX16028)
作者单位
史 莹 南京医科大学附属南京医院(南京市第一医院)呼吸科江苏 南京 210006 
毛 山 南京医科大学附属南京医院(南京市第一医院)呼吸科江苏 南京 210006 
叶 亮 南京医科大学附属南京医院(南京市第一医院)呼吸科江苏 南京 210006 
谷 伟 南京医科大学附属南京医院(南京市第一医院)呼吸科江苏 南京 210006 
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中文摘要:
      目的:检测Kv1.3钾通道在哮喘患者外周血T淋巴细胞中的表达水平,研究Kv1.3钾通道阻滞剂对哮喘患者外周血T淋巴细胞增殖及产生Th2细胞因子的影响,从而探讨Kv1.3钾通道阻滞剂应用于治疗哮喘患者慢性气道炎症的可能。方法:分离哮喘患者及健康对照者的外周血T淋巴细胞,检测T细胞中Kv1.3钾通道的mRNA及蛋白水平,并检测Kv1.3钾通道抑制剂ShK对T细胞增殖及产生Th2细胞因子的作用。结果:哮喘患者外周血T淋巴细胞的Kv1.3水平较健康对照者明显增高,Kv1.3钾通道抑制剂ShK能明显抑制T淋巴细胞的增殖及产生Th2细胞因子的能力。结论:Kv1.3可能是哮喘患者T细胞参与气道炎症的形成及发展的重要蛋白。选择性阻断Kv1.3钾通道可能成为哮喘未来治疗的方向之一。
英文摘要:
      Objective:To detect the expression and function of Kv1.3 channel in peripheral blood T lymphocytes of asthma patients,to study the effect of Kv1.3 channel blockers on the proliferation of T lymphocytes and the production of Th2 cytokines in peripheral blood of asthmatic patients,and to explore the possibility of Kv1.3 channel blockers in treating chronic airway inflammation in patients with asthma. Methods:T lymphocytes were isolated from peripheral blood of asthma patients and healthy controls. Then,we detected the mRNA levels of Kv1.3 in T cells. We detected the effect of Kv1.3 channel inhibitors ShK on T cell proliferation and the production of Th2 cytokines. Results:The Kv1.3 level of peripheral blood T lymphocytes in patients with asthma was significantly higher than that of healthy controls. ShK significantly inhibited the proliferation and Th2 cytokines production ability of T lymphocytes. Conclusion:Kv1.3 may play important roles in inflammation in asthma. Selective blocking of Kv1.3 channels may be one of the future treatments of asthma.
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