文章摘要
张丽娜,周蓓蓓,来艺青,强 贤,冒志磊.高危型HPVE6/E7 mRNA检测在HPV DNA阳性患者中的临床意义[J].南京医科大学学报,2018,(10):1401~1403,1420
高危型HPVE6/E7 mRNA检测在HPV DNA阳性患者中的临床意义
Clinical significance of high⁃risk HPV E6/E7 mRNA test in patients with HPV infection
投稿时间:2018-02-05  
DOI:10.7655/NYDXBNS20181014
中文关键词: 高危型HPVE6/E7 mRNA检测  HPV DNA检测  临床意义
英文关键词: HR⁃HPV E6/E7 mRNA test  HPVDNA test  clinical value
基金项目:江苏省青年医学重点人才资助项目(QNRC2016303);南京医科大学科技发展基金重点项目(2017NJMUZD045)
作者单位
张丽娜 南京医科大学附属常州妇幼保健院宫颈疾病诊治中心江苏 常州 213003 
周蓓蓓 南京医科大学附属常州妇幼保健院宫颈疾病诊治中心江苏 常州 213003 
来艺青 南京医科大学附属常州妇幼保健院宫颈疾病诊治中心江苏 常州 213003 
强 贤 南京医科大学附属常州妇幼保健院宫颈疾病诊治中心江苏 常州 213003 
冒志磊 南京医科大学附属常州妇幼保健院宫颈疾病诊治中心江苏 常州 213003 
摘要点击次数: 173
全文下载次数: 154
中文摘要:
      目的:探讨高危型人乳头状瘤病毒(high risk human papilloma virus,HR?HPV)E6/E7 mRNA检测在HPV病毒感染患者中的临床价值和意义。方法:选取2017年1—6月在宫颈疾病诊治中心进行宫颈机会性筛查,且HPV DNA检测阳性病例200例,分别行HPVE6/E7 mRNA检测和阴道镜下的宫颈活组织检查,分析病毒阳性患者中E6/E7 mRNA的检出率以及不同病理诊断中E6/E7 mRNA的表达情况。结果:200例HPV DNA阳性患者中,HPVE6/E7 mRNA检出率为60.0%。病理学诊断高度鳞状上皮内病变(HSIL)以上,E6/E7 mRNA阳性表达最高,与正常组或低度鳞状上皮内病变(LSIL)组均有明显差异,并且E6/E7 mRNA检测的敏感度和阴性预测值较高,分别为97.9%和98.8%(P < 0.05)。病理学诊断≤LSIL,E6/E7 mRNA阳性率较低,敏感度、特异度、阳性预测值和阴性预测值都不高。结论:HPVE6/E7 mRNA基因检测可用于HPV DNA阳性患者的分流,避免过度治疗,减轻患者心理负担。HPVE6/E7 mRNA阳性表达在宫颈筛查中对高级别病变的诊断具有临床价值。
英文摘要:
      Objective:To investigate the clinical value and significance of high risk human papilloma virus(HPV)E6/E7 mRNA test in patients with HPV infection. Methods:Between January 2017 and June 2017,in Center of Diagnosis and Treatment for Cervical Disease,200 patients with HPV infection in cervical screening received high?risk HPV E6/E7 mRNA test and pathological biopsy under colposcopy. We analyzed the E6/E7 mRNA detection rate of positive cases,and the E6/E7mRNA expression in different pathological diagnosis. Results:The E6/E7 mRNA detection rate was 60% in the 200 patients. The highest E6/E7 mRNA positive expression in the high?grade squamous intraepithelial lesion group(HSIL)had significant differences with the low?grade squamous intraepithelial lesion group(LSIL)and the nomal group. The mRNA sensitivity was 97.9% and negative predictive value was 98.8%(P < 0.05). The sensitivity,specificity,positive predictive value and negative predictive value were lower in the LSIL group. Conclusion:We could shunt the patients with HPV infection,avoid excessive treatment and reduce the patient’s psychological burden byHR?HPV E6/E7 mRNA test. High?risk HPV E6/E7 mRNA test has high clinical value in diagnosis of HSIL during cervical screening.
查看全文   查看/发表评论  下载PDF阅读器
关闭