文章摘要
许云仙,金 蕊,陈筱青,何思思,胡毓华.miR⁃21在早产儿呼吸窘迫综合征患儿血清中表达水平的研究[J].南京医科大学学报,2018,(10):1409~1412
miR⁃21在早产儿呼吸窘迫综合征患儿血清中表达水平的研究
Serum miR⁃21 expression level in infant respiratory distress syndrome
投稿时间:2018-02-28  
DOI:10.7655/NYDXBNS20181016
中文关键词: miR⁃21  呼吸窘迫综合征  早产儿
英文关键词: miR⁃21  infant respiratory distress syndrome  premature infant
基金项目:国家自然科学基金青年基金(81300023,81300521);江苏省自然科学基金青年基金 (BK20131020);江苏高校优势学科建设工程(JX10231081);江苏省妇幼保健重点学科(FXK 201730)
作者单位
许云仙 南京医科大学第一附属医院儿科江苏 南京 210029 
金 蕊 南京医科大学第一附属医院儿科江苏 南京 210029 
陈筱青 南京医科大学第一附属医院儿科江苏 南京 210029 
何思思 南京医科大学第一附属医院儿科江苏 南京 210029 
胡毓华 南京医科大学第一附属医院儿科江苏 南京 210029 
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中文摘要:
      目的:探讨早产儿呼吸窘迫综合征(respiratory distress syndrome,RDS)患儿外周血血清中miR?21的表达水平及其临床意义。方法:选取2015年6—12月入住南京医科大学第一附属医院新生儿监护病房的RDS患儿30例;对照组为非RDS早产儿共21例,使用逆转录PCR法检测外周血血清中miR?21的表达水平,并分析各组病例的临床特点。结果:RDS患儿血清中miR?21表达水平明显高于对照组,差异有统计学意义(P < 0.05)。中重度RDS组miR?21表达水平有明显增加趋势,但目前尚未发现差异有统计学意义。RDS患儿中使用肺表面活性物质(pulmonary surfactant,PS)组miR?21表达水平明显增加(P < 0.05)。结论:血清miR?21表达水平与RDS密切相关,提示miR?21在RDS发病机制中起一定作用。
英文摘要:
      Objective:To determine the pathological significance of serum miR?21 expression level in premature respiratory distress syndrome(RDS)infants. Methods:Randomized blood samples were collected from RDS and non?RDS infants in the Neonatal Intensive Care Unit(NICU)of Jiangsu Province Hospital from June to December 2015. Total RNA was extracted from each blood sample and reverse transcribed into complementary DNA(cDNA). We used real?time reverse transcription polymerase chain reaction(RT?PCR)technique to determine the serum miR?21 expression levels in the RDS and the non?RDS groups(n=30 and n=21,respectively). Unpaired student t?test was used to compare the significance between groups. Results:The serum miR?21 expression level in the RDS group was significantly higher than that in the control group(P < 0.05). The miR?21 expression levels in the moderate to severe RDS groups appeared to be higher than that in the mild groups,however,statistic significance was not reached in our study. In addition,the RDS group that received pulmonary surfactant therapy had significant increase in miR?21 expression levels(P < 0.05). Conclusion:Serum miR?21 expression level is closely related to the development of infant RDS,suggesting that miR?21 is involved in the pathogenesis of infant RDS.
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