文章摘要
李 平,徐桂冬,庞 智,翁嘉懿,尹 娟,孙康云.高通量测序分析冠心病患者外周血 LncRNA表达差异[J].南京医科大学学报,2018,(12):1696~1700
高通量测序分析冠心病患者外周血 LncRNA表达差异
Analysis the expression differences of peripheral blood LncRNA in patients with coronary artery disease by high⁃throughput sequencing
投稿时间:2018-08-10  
DOI:10.7655/NYDXBNS20181207
中文关键词: 冠心病  长链非编码 RNA  高通量测序  外周血单核细胞
英文关键词: coronary artery disease  LncRNAs  high⁃throughput sequencing  peripheral blood mononuclear cells
基金项目:苏州市卫生和计划生育委员会临床重点病种诊疗技术专项(LCZX201610)
作者单位
李 平 南京医科大学附属苏州医院中心实验室江苏 苏州 215008 
徐桂冬 南京医科大学附属苏州医院心血管内科江苏 苏州 215008 
庞 智 南京医科大学附属苏州医院中心实验室江苏 苏州 215008 
翁嘉懿 南京医科大学附属苏州医院心血管内科江苏 苏州 215008 
尹 娟 南京医科大学附属苏州医院中心实验室江苏 苏州 215008 
孙康云 南京医科大学附属苏州医院心血管内科江苏 苏州 215008 
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中文摘要:
      目的:发现外周血中与冠状动脉粥样硬化性心脏病(冠心病)相关的未知长链非编码RNA(LncRNA)。方法:选取临床冠心病组及对照组各3例外周血血浆样本进行LncRNA高通量测序表达谱分析,使用cuffdiff软件获得LncRNA表达谱,计算两组样本间的倍数变化和P?value值,筛选差异表达LncRNA。Q?PCR法验证36对冠心病和正常组外周血浆及外周血单核细胞中的基因表达水平。结果:测序结果分析发现差异表达明显的45个上调表达及29个下调表达的LncRNA。Q?PCR法筛选出3个在冠心病患者外周血单核细胞中上调表达明显的LncRNA:uc003pxg.1,ENST00000565257,ENST00000568324。ROC曲线下面积分别为0.812 5,0.742 4,0.742 4。以上基因在冠心病患者中上调表达均差异显著,与测序结果一致。结论:初步从冠心病患者外周血样本中筛选出上调表达的LncRNA,这为后续深入研究奠定基础,为LncRNA用于冠心病临床早期筛查提供理论依据。
英文摘要:
      Objective:To discover unknown LncRNAs associated with Coronary Artery Disease(CAD)in peripheral blood. Methods:Three pairs of clinical CAD group and control blood plasma samples were texted in this experiment by high?throughput sequencing methods. Cuffdiff software was used to get the expression profiles of LncRNAs,differentially expressed LncRNAs were identified based on fold change and p?value. Differently expressed genes in peripheral plasma and blood monocytes were verified by Q?PCR in 36 pairs of clinical CAD group and controls. Results:The optimal 45 up?regulated and 29 down?regulated LncRNAs were detected. Three LncRNAs uc003pxg.1,ENST00000565257,ENST00000568324 were up?regulated obviously in peripheral blood mononuclear cells of CAD patients. Area under the ROC curve results of uc003pxg.1,ENST00000565257,ENST00000568324 were 0.812 5,0.742 4,0.742 4 respectively. These genes were all up?regulated in CAD patients obviously and these outcomes were consistent with the sequencing results. Conclusion:The ideal LncRNAs were detected preliminarily. This founding lays the foundation for further research,and provides theoretical basis of LncRNA using for CAD early clinical screening.
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