文章摘要
权 哲,张蓓蓓,殷 芳,王婷婷,杜霁儒,支媛婷,钱 苗,刘 玮,许 瑾,宋宁静.青蒿琥酯对豚鼠经皮激素诱导后病理性改变的影响[J].南京医科大学学报,2019,(2):201~204
青蒿琥酯对豚鼠经皮激素诱导后病理性改变的影响
Effects of artesunate on pathological changes induced by glucocorticoid in guinea pigs
投稿时间:2018-11-03  
DOI:10.7655/NYDXBNS20190208
中文关键词: 青蒿琥酯  炎症因子  疾病模型  豚鼠
英文关键词: artesunate  inflammatory factors  desease models  guinea pigs
基金项目:上海市长宁区卫计委科研基金(20164Y005);上海市同仁医院院级课题基金(TRYJ201508)
作者单位
权 哲 上海交通大学医学院附属同仁医院皮肤科上海 200336 
张蓓蓓 上海交通大学医学院附属同仁医院皮肤科上海 200336 
殷 芳 上海交通大学医学院附属同仁医院皮肤科上海 200336 
王婷婷 上海交通大学医学院附属同仁医院皮肤科上海 200336 
杜霁儒 上海交通大学医学院附属同仁医院皮肤科上海 200336 
支媛婷 上海交通大学医学院附属同仁医院皮肤科上海 200336 
钱 苗 上海交通大学医学院附属同仁医院皮肤科上海 200336 
刘 玮 上海交通大学医学院附属同仁医院皮肤科上海 200336 
许 瑾 上海交通大学医学院附属同仁医院皮肤科上海 200336 
宋宁静 上海交通大学医学院附属同仁医院皮肤科上海 200336 
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中文摘要:
      目的:观察青蒿琥酯对豚鼠经皮激素诱导后病理性改变及炎症因子的影响。方法:30只雄性豚鼠背腹部剃毛区外用0.05%丙酸氯倍他索溶液复制模型,并将其随机等分为5组:模型组给予0.9%氯化钠溶液腹腔注射,治疗组分别给予低、中、高剂量[5、10、20 mg/(kg·d)]青蒿琥酯注射液腹腔注射,糖皮质激素受体阻断组给予米非司酮腹腔注射;另取6只豚鼠作为空白对照组,仅剃毛区外涂75%乙醇溶液。观察各组皮肤组织病理学改变,ELISA法检测各组血清中白细胞介素(interleukin,IL)?1、IL?4含量,采用RT?PCR检测各组皮肤中糖皮质激素受体α(GRα)和肿瘤坏死因子(tumor necrosis factor,TNF)?α表达。结果:模型组血清IL?1、IL?4含量明显高于空白对照组和糖皮质激素受体阻断组(P<0.05),青蒿琥酯腹腔注射中、高剂量组血清中IL?1、IL?4含量均低于模型组(P<0.05),治疗组间比较差异无统计学意义。各组豚鼠皮肤组织GRa mRNA的表达差异无统计学意义。模型组皮肤组织TNF?α mRNA含量高于空白对照组,青蒿琥酯腹腔注射中、高剂量组皮肤组织TNF?α mRNA含量低于模型组(P<0.05),中、高剂量组间比较差异无统计学意义。结论:青蒿琥酯能抑制豚鼠类固醇诱导的皮炎,以中、高剂量最为明显,其作用可能与IL?1、IL?4、TNF?α有关,具体机制有待进一步深入研究。
英文摘要:
      Objective:To observe the effect of artesunate on pathological changes and inflammatory factors induced by glucocorticoid in guinea pig models. Methods:Thirty male guinea pigs were used 0.05% clobetasol propionate solution continuously on the shaved belly areas for 30 days to establish guinea pig models of steroid?induced dermatitis. They were randomly and equally divided into 5 groups:model groups were intraperitoneal injected with 0.9% sodium chloride solution,treatment groups were intraperitoneal injected with 5,10 and 20 mg/kg artesunate solution separately,glucocorticoid receptor blocking groups were intraperitoneal injected with mifepristone,and 6 guinea pigs only external used with 75% ethanol solution on shaving back as blank control groups. Pathological changes in skin tissue of each group were observed. Enzyme?linked immunosorbent assay(ELISA)was perfomed to detect the content of IL?1 and IL?4 in the serum of each group. The expressions of GRα mRNA and TNF?α mRNA in the skin of each group were detected by RT?PCR. Results:The levels of IL?1 and IL?4 in the serum of model groups were significantly higher than those in the blank control groups and the glucocorticoid receptor blocked groups. The levels of IL?1 and IL?4 in the serum of artesunate groups were lower than those in the model group(P < 0.05). There was no significant difference between the treatment groups. There was no significant difference in the expression of GRa mRNA in the skin tissues of guinea pigs. The TNF?α mRNA content in the skin tissue of the model group was higher than that in the blank control group and the glucocorticoid receptor blocked group(P < 0.05). The content of TNF?α mRNA in the skin tissue of the high dose and middle dose group of artesunate was lower than that of the model group(P < 0.05). There was no significant difference in TNF?α mRNA between the middle dose artesunate group and the high dose arteunate group. Conclusion:Artesunate can inhibit steroid dermatitis in guinea pig models,especially the middle and high dose artesunate,which may associate with IL?1,IL?4 and TNF?α,and the mechanism needs to be further studied.
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