Objective：To analyze the clinical effect of single kidney from pediatric donors ≤ 5 years of age in adult recipients transplantation. Methods：A retrospective study was conducted to analyze the clinical data of nine adult recipients recieving single kidney from pediatric donors from November 2013 to September 2016 in the First Affiliated Hospital with Nanjing Medical University. Results：Six adult recipients received single kidney from five pediatric donors ≤ 5 years of age. For the donors，the median age was 50 months（range，20 to 60 months）and the median weight was 15.5 kg（range，12.0 to 17.0 kg）. Six recipients were female，with their median age 25 years（range，18~31 years），their median weight 43.5 kg（range，34.0~55.0 kg），their median BMI 18.3（range，14.7~22.0）. During the follow-up period，the recipients’ serum creatinine level gradually decreased，and their eGFR increased steadily. The renal function stayed stable within six months to three years after transplantation. The long diameter of the transplanted kidney grew rapidly within 2 weeks after operation（P=0.042），and further growth occurred within 1 year after operation. The 1-year patient/graft survival was 100%. Conclusion：Single renal supply from pediatric donors ≤ 5 years of age is feasible and effective for adult recipients transplantation.