文章摘要
刘 玲,钱潇潇,宋 斌,谭 娟,窦开琴,侯大卫,尤 强,丁大法,鲁一兵.lncRNA TCONS⁃00020455及TCONS⁃00020457在甲状腺乳头状癌中的表达及意义[J].南京医科大学学报,2019,(3):332~337
lncRNA TCONS⁃00020455及TCONS⁃00020457在甲状腺乳头状癌中的表达及意义
Expression and significance of lncRNA TCONS⁃00020455 and TCONS⁃00020457 in papillary thyroid carcinoma
投稿时间:2018-04-10  
DOI:10.7655/NYDXBNS20190304
中文关键词: PTC  长链非编码RNA  表达  转移
英文关键词: thyroid papillary carcinoma  long non⁃coding RNA  expression  metastasis
基金项目:江苏省重点研发计划(社会发展)重点项目(BE2015723)
作者单位
刘 玲 南京医科大学第二附属医院内分泌科江苏 南京 210011 
钱潇潇 南京医科大学第二附属医院内分泌科江苏 南京 210011 
宋 斌 南京医科大学第二附属医院内分泌科江苏 南京 210011 
谭 娟 南京医科大学第二附属医院内分泌科江苏 南京 210011 
窦开琴 南京医科大学第二附属医院内分泌科江苏 南京 210011 
侯大卫 南京医科大学第二附属医院内分泌科江苏 南京 210011 
尤 强 南京医科大学第二附属医院内分泌科江苏 南京 210011 
丁大法 南京医科大学第二附属医院内分泌科江苏 南京 210011 
鲁一兵 南京医科大学第二附属医院内分泌科江苏 南京 210011 
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中文摘要:
      目的:探讨长链非编码RNA(long non?coding RNA,lncRNA)在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)组织中表达量的变化和lncRNA在PTC发生发展中的作用。方法:应用微阵列芯片技术检测PTC手术切除的癌组织及癌旁组织中的lncRNA表达谱,并从中随机选取5条差异表达的lncRNA(Fold change≥2,P < 0.05),应用real?time PCR方法验证表达谱芯片结果。比较不同表达水平lncRNA与PTC患者临床特征、疾病分期之间的关系。结果:基因芯片结果显示,lncRNA在PTC组织及癌旁组织中的表达具有明显差异性,429个表达上调,1 449个表达下调。在41例PTC患者癌组织及癌旁组织中,real?time PCR检测结果显示,lncRNA TCONS?00020455(TCON?455)及TCONS?00020457(TCON?457)在PTC癌组织中的表达水平均显著高于癌旁组织,差异均具有统计学意义(P < 0.05)。进一步分析发现,lncRNA TCON?455及TCON?457表达水平与PTC患者淋巴结转移、多发病灶和TNM分期相关(P均<0.05),而与年龄、性别均无相关性(P均>0.05)。结论:PTC患者的癌组织中存在lncRNA异常表达。lncRNA TCON?455和TCON?457的高表达可能与PTC转移和恶化有关。
英文摘要:
      Objective:To investigate changes of long non?coding RNA expression in papillary thyroid carcinoma(PTC)and the role of lncRNA in occurrence and development of PTC. Methods:The microarray chip technique was used to detect the lncRNA expression profile in cancer tissues and paracancerous tissues after PTC resection. Five randomly expressed lncRNAs were randomly selected(fold change≥2,P < 0.05). The real?time PCR method was used to verify expression microarray results. The different expression levels of lncRNA with the clinical features and stage of disease were compared in PTC patients. Results:Gene chip results showed that the expressions of lncRNAs in PTC tissues and paracancerous tissues were significantly different. There were 429 up?regulated lncRNAs and 1 449 down?regulated lncRNAs. In the tumor tissues and paracancerous tissues of 41 patients with PTC,real?time PCR results showed that expression levels of lncRNA TCONS?00020455 and TCONS?00020457(annotated:lncRNA TCONS?00020455 and TCONS?00020457 are abbreviated as lncRNA TCON?455 and TCON?457,and the short names for these two lncRNAs are used in the article.)in PTC tissues were significantly higher than those in adjacent tissues(P < 0.05). Further analysis found that the expression levels of lncRNA TCON?455 and lncRNA TCON?457 were associated with lymph node metastasis,multiple lesions,and TNM stage in PTC patients(P < 0.05),but not correlated with age and gender(P > 0.05). Conclusion:There is abnormal expression of lncRNAs in cancerous tissues of PTC patients. High expression of lncRNA TCON?455 and TCON?457 may be associated with metastasis and progression of PTC.
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