文章摘要
李 琳,李晓曦,毕欣芸,刘珊珊,戴一凡.n⁃3多不饱和脂肪酸对NOD小鼠T细胞的免疫调节作用[J].南京医科大学学报,2019,(12):1707~1711
n⁃3多不饱和脂肪酸对NOD小鼠T细胞的免疫调节作用
The immunoregulation effect of n⁃3 polyunsaturated fatty acids on T cells in NOD mice
投稿时间:2019-03-09  
DOI:10.7655/NYDXBNS20191201
中文关键词: n⁃3多不饱和脂肪酸  T细胞  NOD小鼠
英文关键词: n⁃3 PUFAs  T cell  NOD mice
基金项目:国家自然科学基金(81570402,81874144)
作者单位
李 琳 南京医科大学江苏省异种移植重点实验室江苏 南京 211166 
李晓曦 南京医科大学免疫学系江苏 南京 211167 
毕欣芸 广东工业大学生物医药学院广州 番禺510006 
刘珊珊 青岛市妇女儿童医院检验科山东 青岛 266001 
戴一凡 南京医科大学江苏省异种移植重点实验室江苏 南京 211166 
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中文摘要:
      目的:探讨n?3多不饱和脂肪酸(n?3 poly unsaturated fatty acid,n?3 PUFA)对NOD小鼠T细胞免疫学功能的影响。 方法: 野生型Balb/c小鼠和已出现典型的Ⅰ型糖尿病症状的NOD小鼠脾 细胞,磁珠分选后获得小鼠CD4+ T细胞,将其分 为4组:野生型 鼠为Wild type组;NOD小鼠分为未处理组、二十二碳六烯 (docosahexaenoic acid,DHA)处理组、二十碳五烯酸(eicosapentaenoic acid,EPA)处理组。DHA、EPA处理24h,PMA和Ionomycin刺激活化后,采用CCK?8法检测T细胞增殖 流式细胞仪检测Th1/Th2极化、ELISA法检测T细胞细胞因子的分 水平变化。结果:DHA、EPA对T细胞增殖具有显著抑制作用,促 NOD小鼠Th2细胞分化,抑制Th1细胞分化,经ELISA检测,DHA EP 能够抑制T细胞IL?6、IL?17的分泌。结论:n?3多不 饱和脂肪酸 过抑制T细胞增殖,平衡Th1/Th2比例和抑制IL?6、IL?17的分泌对NOD小鼠的T淋巴细胞起到免疫抑制作用。
英文摘要:
      Objective:This study aims to observe the effects of n ? 3 poly unsaturated fatty acids(n ? 3 PUFA)on immunological functions of T cells in NOD mice. Methods:Spleen cells were separated from Balb/c WT mice and NOD mice. The CD4+ T cells were obtained from magnetic bead separated spleen cells and divided into four groups:wild type group,NOD mice untreated group(control), NOD mice treated with(docosahexaenoic acid,DHA)and(eicosapentaenoic acid,EPA)groups. The NOD mice T cells were treated with EPA and DHA respectively for 24 h. After the stimulation and activation of PMA and lonomycin,the viability of T cells was tested by CCK?8 kit,the differentiation of Th cells was analyzed by flow cytometer,and the secretion of inflammatory cytokines was measured by ELISA Kits. Results:EPA and DHA could inhibit the viability of T cells from NOD mice,suppress the naive T cells’differentiation into Th1 cells,balance the ratio of Th1 and Th2,and down?regulate the secretion of IL?6 and IL?17. Conclusion:n?3 PUFAs could suppress some immune functions of T cells by inhibiting their viability,regulating the Th1/Th2 polarization and inhibiting the secretion of IL?6 and IL?17.
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