Objective：To investigate the role of long non-coding RNA（lncRNA）SNHG14 in the progression of gastric cancer（GC）. Methods：RT-qPCR was used to detect the expression level of SNHG14 in 68 pairs of gastric cancer tissues and adjacent normal tissues，3 gastric cancer cell lines（MGC-803，BGC-823，SGC-7901）and human normal gastric epithelial cell line GES-1. The correlation between the expression level of SNHG14 and the clinicopathological features in gastric cancer tissues and gastric cancer cell lines was analyzed. The function of gastric cancer cell lines was observed after SNHG14 silencing by siRNA. The effect of SNHG14 on the proliferation of gastric cancer cells was evaluated by means of CCK-8 proliferation assay. Transwell assay was used to detect the migration and invasion of gastric cancer cells. Results：The expression level of SNHG14 was significantly up-regulated in the gastric cancer cell lines and gastric cancer tissues compared with normal controls. Result of correlation analysis showed that the expression level of SNHG14 was significantly correlated with the TNM stage（P < 0.05），degree of differentiation（P < 0.001），tumor size（P < 0.01）and depth of invasion（P < 0.05）of gastric cancer tissues. The SNHG14 silencing by siRNA significantly inhibited the proliferation（P < 0.01），migration（P < 0.01）and invasion（P < 0.05）of gastric cancer cells. Conclusion：SNHG14 may play a role in promoting cancer and affect the progression of gastric cancer by promoting the proliferation and metastasis of gastric cancer cells. SNHG14 bears potential value in the treatment and diagnosis of gastric cancer，and may become a novel biomarker for the diagnosis of gastric cancer or a candidate target in the biotherapy of gastric cancer.