文章摘要
渠辉恒,王 宁,陈义钢,陈 烨,夏加增.长链非编码RNA SNHG14可能通过促进胃癌细胞增殖和转移影响胃癌的进展[J].南京医科大学学报,2020,(1):50~55
长链非编码RNA SNHG14可能通过促进胃癌细胞增殖和转移影响胃癌的进展
The role of long non⁃coding RNA SNHG14 in proliferation,invasion and migration of gastric cancer
投稿时间:2018-11-01  
DOI:10.7655/NYDXBNS20200110
中文关键词: 胃癌  长链非编码RNA  SNHG14  细胞增殖  侵袭  迁移
英文关键词: gastric cancer  long noncoding RNA  SNHG14  cell proliferation  invasion  migration
基金项目:无锡市医学杰出人才培育对象(JCRCPY001)
作者单位
渠辉恒 南京医科大学附属无锡第二医院普外科江苏 无锡 214000 
王 宁 南京医科大学附属无锡第二医院普外科江苏 无锡 214000 
陈义钢 南京医科大学附属无锡第二医院普外科江苏 无锡 214000 
陈 烨 南京医科大学附属无锡第二医院普外科江苏 无锡 214000 
夏加增 南京医科大学附属无锡第二医院普外科江苏 无锡 214000 
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中文摘要:
      目的:研究长链非编码RNA(long non?coding RNA,lncRNA)SNHG14在胃癌(gastric cancer,GC)进展中的作用。方法:应用RT?qPCR检测SNHG14在68对胃癌组织和癌旁组织、3个胃癌细胞系(MGC?803、BGC?823、SGC?7901)和人类正常胃上皮细胞株GES?1中的表达量。分析胃癌组织及胃癌细胞株内的SNHG14表达水平与临床病理特征的相关性。使用siRNA沉默SNHG14后观察胃癌细胞株的功能。CCK?8增殖实验评估SNHG14对胃癌细胞增殖的影响,Transwell实验检测胃癌细胞迁移和侵袭能力。结果:胃癌细胞株和胃癌组织中SNHG14的表达量与正常对照相比显著上调。相关分析显示SNHG14的表达量与胃癌组织的TNM分期(P < 0.05)、分化程度(P < 0.001)、肿瘤大小(P < 0.01)及浸润深度(P < 0.05)等有显著相关性。通过siRNA沉默SNHG14的表达后,能够显著抑制胃癌细胞的增殖(P < 0.01)、迁移(P < 0.01)和侵袭(P < 0.05)能力。结论:SNHG14可能起到促癌作用,通过促进胃癌细胞的增殖和转移来影响胃癌的进展。SNHG14在治疗和诊断胃癌中具有潜在价值,有可能成为诊断胃癌的新型生物标志物或胃癌生物治疗的候选靶点。
英文摘要:
      Objective:To investigate the role of long non?coding RNA(lncRNA)SNHG14 in the progression of gastric cancer(GC). Methods:RT?qPCR was used to detect the expression level of SNHG14 in 68 pairs of gastric cancer tissues and adjacent normal tissues,3 gastric cancer cell lines(MGC?803,BGC?823,SGC?7901)and human normal gastric epithelial cell line GES?1. The correlation between the expression level of SNHG14 and the clinicopathological features in gastric cancer tissues and gastric cancer cell lines was analyzed. The function of gastric cancer cell lines was observed after SNHG14 silencing by siRNA. The effect of SNHG14 on the proliferation of gastric cancer cells was evaluated by means of CCK?8 proliferation assay. Transwell assay was used to detect the migration and invasion of gastric cancer cells. Results:The expression level of SNHG14 was significantly up?regulated in the gastric cancer cell lines and gastric cancer tissues compared with normal controls. Result of correlation analysis showed that the expression level of SNHG14 was significantly correlated with the TNM stage(P < 0.05),degree of differentiation(P < 0.001),tumor size(P < 0.01)and depth of invasion(P < 0.05)of gastric cancer tissues. The SNHG14 silencing by siRNA significantly inhibited the proliferation(P < 0.01),migration(P < 0.01)and invasion(P < 0.05)of gastric cancer cells. Conclusion:SNHG14 may play a role in promoting cancer and affect the progression of gastric cancer by promoting the proliferation and metastasis of gastric cancer cells. SNHG14 bears potential value in the treatment and diagnosis of gastric cancer,and may become a novel biomarker for the diagnosis of gastric cancer or a candidate target in the biotherapy of gastric cancer.
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