文章摘要
荣家闵,于 明,葛雅琨,张仁文,劳 军,张元新.水飞蓟宾对H2O2诱导SH⁃SY5Y细胞损伤的保护作用及机制[J].南京医科大学学报,2020,(4):515~520
水飞蓟宾对H2O2诱导SH⁃SY5Y细胞损伤的保护作用及机制
Protective effects and mechanism of silibinin on SH⁃SY5Y cell injury induced by hydrogen peroxide
投稿时间:2019-06-13  
DOI:10.7655/NYDXBNS20200410
中文关键词: 水飞蓟宾  氧化应激  H2O2  帕金森病
英文关键词: silibinin  oxidative stress  H2O2  Parkinson’s disease
基金项目:吉林省省级产业创新专项资金(2018C049?4);吉林省教育厅科研项目 [吉教科合字(2016)第133号];吉林市科技局项目(201750259);吉林化工学院校级重大项目(20180101)
作者单位
荣家闵 吉林化工学院生物与食品工程学院吉林 吉林 132022 
于 明 吉林化工学院生物与食品工程学院吉林 吉林 132022 
葛雅琨 吉林化工学院生物与食品工程学院吉林 吉林 132022 
张仁文 吉林化工学院生物与食品工程学院吉林 吉林 132022 
劳 军 吉林化工学院生物与食品工程学院吉林 吉林 132022 
张元新 吉林化工学院生物与食品工程学院吉林 吉林 132022 
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中文摘要:
      目的:探究水飞蓟宾对过氧化氢(H2O2)诱导SH?SY5Y细胞损伤的保护作用及可能机制。方法:采用体外培养方法,建立SH?SY5Y细胞的H2O2损伤模型。用水飞蓟宾预处理细胞后MTT法测定细胞的存活率;流式细胞术检测活性氧(reactive oxygen species,ROS)、细胞凋亡和线粒体膜电位的变化;Western blot法检测Cleaved?Caspase?9、Cleaved?Caspase?3蛋白表达情况。结果:与模型组比较,用50和100 μmol/L水飞蓟宾预处理SH?SY5Y细胞3 h能明显减轻H2O2诱导的SH?SY5Y损伤,显著提高细胞存活率(P < 0.01)和减少凋亡(P < 0.05),减少细胞内ROS水平,稳定线粒体膜电位(P < 0.05),使Cleaved?Caspase?9和Cleaved?Caspase?3的表达量下降。结论:水飞蓟宾可以保护SH?SY5Y细胞免受H2O2的损伤,其机制可能与水飞蓟宾的抗氧化性、保护线粒体膜电位和降低Cleaved?Caspase?9和Cleaved?Caspase?3的表达量有关。
英文摘要:
      Objective:To study the protective effect and possible mechanism of silibinin on cell damage of SH?SY5Y induced by hydrogen peroxide(H2O2). Methods:SH?SY5Y cell damage model was established by H2O2 in vitro. After pretreatment with silibinin,the cell viability was determined by MTT,reactive oxygen species(ROS),apoptosis and mitochondrial membrane potential(MMP)were determined by flow cytometry,and the expressions of Cleaved?Caspase?9 and Cleaved?Caspase?3 were detected by Western blot. Results:Compared to the model group,pretreatment with 50 μmol/L and 100 μmol/L silibinin for 3 h attenuated the damage of SH?SY5Y cells treated with H2O2,improved the cell survival rate(P<0.05),reduced the apoptosis rates(P<0.05)and the release of ROS,stabilized MMP(P<0.01),decreased the expression of Cleaved?Caspase?9 and Cleaved?Caspase?3. Conclusion:Silibinin can protect SH?SY5Y cell from H2O2,which may be related to its antioxidant capacity,protection of mitochondrial membrane potential,and reduction of the expression of Cleaved?Caspase?9 and Cleaved?Caspase?3.
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