Objective：To investigate whether gelatin methacrylamide（GelMA）scaffolds made by low-temperature 3D printing could construct high-quality tissue-engineered cartilage. Methods：After pre-cooling，low-temperature deposition 3D printing technology was used to prepare low-concentration gelatin methacrylamide scaffolds. Macroscopic and scanning electron microscopic observation of the macroscopic and microscopic structure of the scaffold were followed by a rheological and biomechanical detection of the hydrogel. Cells were cultured with DMEM as control，cell proliferation（CCK-8 method）and cell activity（Live/Dead staining method）were used to detect the biocompatibility of the scaffold，and histological staining was used to qualitatively analyze the scaffold components. Nude mice were scaffolded subcutaneously for hematoxylin and eosin（HE）staining，alcian blue staining，and safranin staining to highlight the extracellular matrix structure of the cartilage. Results：Macroscopic and scanning electron microscopic observations showed that the 5% GelMA scaffolds had uniform pore diameters and a regular structure. The results of CCK-8 and live/dead cell staining showed that the scaffolds were biocompatible，and the in vivo and in vitro histological staining showed that the constructed tissue-engineered cartilage met the requirements in colors，glycosaminoglycan（sGAG）secretion and other aspects. Conclusion：5% GelMA bio-ink can be used for 3D printing at low temperature and to successfully construct tissue-engineered cartilage.