文章摘要
张 明,王吉荣.miR⁃552⁃3p通过抑制DACH1促进肝细胞性肝癌细胞增殖、迁移和侵袭[J].南京医科大学学报,2020,(4):538~544
miR⁃552⁃3p通过抑制DACH1促进肝细胞性肝癌细胞增殖、迁移和侵袭
MiR⁃552⁃3p promotes hepatocellular carcinoma cell proliferation,migration and invasion by inhibiting DACH1
投稿时间:2019-05-26  
DOI:10.7655/NYDXBNS20200414
中文关键词: miR⁃552⁃3p  DACH1  肝细胞性肝癌
英文关键词: miR⁃552⁃3p  DACH1  hepatocellular carcinoma
基金项目:
作者单位
张 明 南京医科大学附属江宁医院普外科江苏 南京 211100 
王吉荣 南京医科大学附属江宁医院普外科江苏 南京 211100 
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中文摘要:
      目的:探讨miR?552?3p在肝细胞性肝癌(hepatocellular carcinoma,HCC)中的表达及其促进细胞增殖和迁移、侵袭的机制。方法:qRT?PCR检测HCC细胞系及人正常肝细胞系中miR?552?3p的表达情况;下载TCGA数据库中HCC样本的微阵列数据,分析miR?552?3p在HCC癌组织及癌旁组织中是否存在表达差异;miR?552?3p mimics以及miR?552?3p inhibitor转染HCC细胞,荧光素酶报告基因验证miR?552?3p与DACH1是否存在靶向关系,CCK?8及Transwell实验检测miR?552?3p及其与DACH1相互作用对于HCC细胞增殖、迁移、侵袭的影响。结果:TCGA数据库中HCC样本的分析结果表明,miR?552?3p在HCC中高表达,同时qRT?PCR显示miR?552?3p在HCC细胞系中的表达水平显著高于人肝细胞系L02;miR?552?3p的高表达促进HCC细胞的增殖、迁移和侵袭,而抑制miR?552?3p表达后则相反;荧光素酶报告实验证实miR?552?3p靶向作用于DACH1的3′?UTR,上调miR?552?3p可抑制DACH1的表达,而下调miR?552?3p则增加DACH1表达;DACH1的过表达可抑制HCC细胞增殖、迁移和侵袭,但同时过表达miR?552?3p后DACH1对于HCC细胞相关功能的抑制即可解除;miR?552?3p正向调控Wnt/β?catenin信号通路的激活。结论:miR?552?3p靶向作用于DACH1促进HCC细胞增殖、迁移和侵袭,并参与调控Wnt/β?catenin信号。可能为HCC的诊疗策略提供潜在靶点。
英文摘要:
      Objective:To investigate the expression of miR?552?3p in hepatocellular carcinoma(HCC)and its mechanism of promoting cell proliferation,migration and invasion. Methods:qRT?PCR was used to detect the expression of miR?552?3p in HCC cell lines and normal human liver cell lines. The microarray data of HCC samples were downloaded from TCGA database,and the expressions of miR?552?3p in HCC tumor tissues and its adjacent tissues were analyzed to show whether they are different or not;miR?552?3p mimics and miR?552?3p inhibitor transfected HCC cells,and luciferase assays verified whether miR?552?3p targets DACH1,while CCK8 and Transwell assays detected the effect of miR?552?3p and its interaction with DACH1 on HCC cell proliferation,migration,and invasion. Results:The analysis of HCC samples from the TCGA database indicated that miR?552?3p was highly expressed in HCC,and qRT?PCR showed that the expression level of miR?552?3p in HCC cell line was significantly higher than that in human liver cell line L02;the high expression of miR?552?3p promoted the proliferation,migration and invasion of HCC cells,while the inhibition of miR?552?3p reversed these functions;the luciferase reporter assays verified that miR?552?3p targets the 3′?UTR of DACH1. Up?regulation of miR?552?3p expression inhibited DACH1 whereas down?regulation of miR?552?3p increased DACH1 expression;DACH1 overexpression inhibited HCC cell proliferation,migration and invasion,but with miR?552?3p overexpression the DACH1 inhibitions of HCC cell?related functions were relieved;miR?552?3p positively regulated the activation of Wnt/β?catenin signaling pathway. Conclusion:miR?552?3p targeting DACH1 promotes HCC cell proliferation,migration and invasion,and regulates Wnt/β?catenin signaling,which may provide potential targets for HCC diagnosis and treatment strategies.
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