文章摘要
杨祖坤,贾 璐,韩利萍,孙柳絮,郭 宇,夏 阳.3D打印PLGA支架修复大鼠上颚骨缺损的实验研究[J].南京医科大学学报,2020,(8):1125~1129
3D打印PLGA支架修复大鼠上颚骨缺损的实验研究
Effects of PLGA scaffold fabricated by 3D printing on the repair of rat palate bone defect
投稿时间:2019-11-10  
DOI:10.7655/NYDXBNS20200808
中文关键词: 3D打印  PLGA支架  骨缺损  骨再生
英文关键词: 3D printing  PLGA scaffold  bone defect  bone regeneration
基金项目:国家自然科学基金(81771044);东南大学?南京医科大学合作研究项目(2242018K3DN16);江苏省青蓝工程项目,江苏省青年医学人才(QNRC2016853);江苏高校优势学科建设工程资助项目(2018?87)
作者单位
杨祖坤 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
贾 璐 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
韩利萍 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
孙柳絮 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
郭 宇 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
夏 阳 南京医科大学口腔疾病研究江苏省重点实验室江苏 南京 210029 
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中文摘要:
      目的:探讨3D打印聚乳酸?羟基乙酸共聚物(poly lactic?co?glycolic acid,PLGA)支架修复大鼠上颚骨缺损的效果。方法:将PLGA 溶解于丙酮中,利用3D打印机打印出间距为100 μm、单层厚度为60 μm的圆盘形网格状支架材料。将12只大鼠随机分为2组(n=6),制备直径为3 mm的上颚圆形骨缺损,实验组在骨缺损中植入3D打印的PLGA支架后缝合关闭缺损,对照组术后仅缝合关闭缺损。术后8周处死大鼠,行micro?CT及组织学检测观察缺损处骨组织修复情况。结果:成功制备3D打印PLGA支架。大鼠上颚骨缺损造模后8周,植入3D打印PLGA支架的实验组骨体积分数(bone?volume/total?volume,BV/TV)大于对照组(P < 0.05),骨缺损未愈合面积小于对照组(P < 0.05)。通过上颚骨组织HE染色,发现实验组骨缺损边缘有大量新生骨质,而对照组只有少量新生骨质。结论:3D打印PLGA支架能够促进大鼠上颚骨缺损的骨再生。
英文摘要:
      Objective:To observe the effects of 3D?printed poly lactic?co?glycolic acid(PLGA)scaffold on the repair of rat palate bone defect. Methods:The PLGA was dissolved in acetone,and disc?shaped mesh scaffolds having pores with diameters of 100 μm and a single layer thickness of 60 μm were printed using a 3D printer. Twelve rats were randomly divided into two groups(n=6)to create round palate bone defects with a diameter of 3 mm. The bone defect in the experimental group was implanted with a 3D?printed PLGA scaffold and sutured to close the defect,while in the control group the defect was sutured to close only. The rats were sacrificed after 8 weeks,and the bone defect repair was evaluated by using micro?CT and histological analysis. Results:3D?printed PLGA scaffolds were successfully fabricated. Eight weeks after the establishment of the palate defects model in rats,the bone?volume/total?volume(BV/TV)of the experimental group implanted with the 3D?printed PLGA scaffold was significantly higher than the control group(P < 0.05),and the unhealed area of the bone defect was also smaller than that of the control group(P < 0.05). HE staining of the palate bone tissue showed that a large amount of new bone was formed at the edge of the defect in the experimental group,while the control group had only a small amount of new bone. Conclusion:3D?printed PLGA scaffold can promote the bone regeneration in palate bone defect.
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