Roles of small RNA 2’⁃O⁃methyltransferase Henmt1 during mouse spermatogenesis
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摘要:
目的：通过构建基因突变小鼠模型，研究小RNA 2’-O-甲基转移酶Henmt1在小鼠精子发生中的作用。方法：利用CRISPR/Cas9（clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9）技术，构建Henmt1基因突变小鼠，通过计算机辅助精子分析、HE染色及免疫荧光检测等技术对小鼠表型进行分析。结果：成功构建Henmt1基因突变小鼠模型；生育力测试结果提示Henmt1基因突变雄鼠不育；Henmt1基因突变导致雄性小鼠精子发生阻滞；突变小鼠睾丸中逆转座子元件LINE1表达上调。结论：Henmt1对雄性小鼠精子发生有重要调控作用。
Abstract:
Objective：This study aims to observe the role of small RNA 2’-O-methyltransferase Henmt1 during mouse spermatogenesis by generation of Henmt1 mutant mouse model. Methods：Henmt1 mutant mouse were constructed by CRISPR/Cas9 system（clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9）and analyzed the phenotype of mutant mice by computer-aided sperm analysis，HE staining and immunofluorescent staining. Results：Henmt1 mutant mouse model were successfully generated. Henmt1 mutant males are sterility with spermatogenic arrest and up-regulation of retrotransposon LINE1. Conclusion：This study found that HENMT1 plays an important regulatory role during spermatogenesis.
