Risk assessment of arrhythmia induced by periplocin in vitro
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摘要:
目的：评价传统中草药香加皮的主要活性成分杠柳毒苷致心律失常的风险。方法：采用离体组织（豚鼠乳突肌和兔浦肯野纤维），给予浓度为0、0.3、1.0、3.0 μmol/L杠柳毒苷，检测其对动作电位的影响。指标包括：静息电位（resting membrane potential，RMP）、动作电位幅度（action potential amplitude，APA）、最大除极速度（maximal depolarization rate of phase 0，dv/dtmax）、复极30%、50%和90%动作电位时程（action potential duration at 30%，50% and 90% repolarization，APD30、APD50、APD90）。结果：与0 μmol/L组比较，0.3和1.0 μmol/L 杠柳毒苷可缩短豚鼠乳突肌的APD30和APD50；0.3、1.0、3.0 μmol/L杠柳毒苷降低兔浦肯野纤维的dv/dtmax；3.0 μmol/L杠柳毒苷减少兔浦肯野纤维的APA；1.0、3.0 μmol/L杠柳毒苷缩短兔浦肯野纤维的APD30。差异均有统计学意义。结论：杠柳毒苷有潜在诱导心律失常的风险。
Abstract:
Objective：The aim of the study was to evaluate the risk of arrhythmia induced by periplocin，the main active component of Cortex Periplocae，a traditional Chinese medicine preparation. Methods：In this experiment，isolated tissues（guinea pig papillary muscle and rabbit Purkinje fiber）were treated by 0.3，1.0，or 3.0 μmol/L periplocin to assess the effect on action potential. The following parameters and endpoints were assessed：resting membrane potential（RMP），action potential amplitude（APA），maximal depolarization rate of phase 0（dv/dtmax），action potential duration at 30%，50%，and 90% repolarization（APD30，APD50，and APD90）. Results：As compared to the 0 μmol/L group，periplocin-related significant reduction in APD30，APD50 was observed at guinea pig papillary muscle in the 0.3 or 1.0 μmol/L periplocin. Periplocin-related significant decrease in dv/dtmax was observed at rabbit Purkinje fiber in the 0.3，1.0，or 3.0 μmol/L periplocin. Periplocin-related significant reduction in APA was observed at rabbit Purkinje fiber in the 3.0 μmol/L periplocin. Periplocin-related significant reduction in APD30 was observed at rabbit Purkinje fiber in the 1.0，or 3.0 μmol/L periplocin. Conclusion：Periplocin has the potential to induce arrhythmia.
