Objective：This study aims to analyze susceptibility and homology of clinically isolated carbapenem-resistant Klebsiella pneumoniae（CRKP）from ICU ward. Methods：A total of 171 independent CRKP isolates were collected between March 2018 and September 2018 from the First Affiliated Hospital of Henan University of Science and Technology. The isolates were identified by MALDI-TOF MS，characterized by agar dilution，microdilution methods and multilocus sequence typing（MLST）. Carbapenemase phenotype was confirmed by mCIM and eCIM test. β-lactamases and serotype were detected by polymerase chain reaction and DNA sequencing. Results：There were 68（39.8%，68/171）CRKP isolates in the hospital and 46（67.6%，46/68）isolates of CRKP in ICU. The resistant rate of the 46 isolates to ceftazidime/avibactam，tigecycline，colistin，and minocycline were 0，0，4.3%，and 41.7%. MIC90 of meropenem，imipenem，ceftazidime，chloramphenicol，fosfomycin，and aztreonam were over 128. Sequence type（ST）11（43/46，93.5%）were predominant. K47（60.9%，28/46）was the most common serotype，followed by K64（23.9%，11/46）. The isolates all harbored β-lactamases of blaKPC-2，blaSHV，and blaTEM，and harbored blaCTX-M （40/46，86.96%），blaDHA（6/46，13.04%）. Conclusion：Most CRKP in ICU are ST11，which co-carried blaKPC-2，blaSHV，blaTEM，and blaCTX-M. All isolates showed serious antimicrobial resistance. Nosocomial infection control may be should focus on preventing CRKP transfer in ICU.