文章摘要
吴莉莉,薛 路,章 浩,乌 兰,丁虹娟.lncRNA HAND2⁃AS1对子痫前期患者血管内皮细胞的影响[J].南京医科大学学报,2020,(12):1761~1767
lncRNA HAND2⁃AS1对子痫前期患者血管内皮细胞的影响
Effects of incRNA HAND2⁃AS1 on vascular endothelial cells in preeclampsia patients
投稿时间:2020-05-30  
DOI:10.7655/NYDXBNS20201204
中文关键词: 子痫前期  长链非编码RNA  人脐静脉内皮细胞
英文关键词: preeclampsia  long non⁃coding RNA  human umbilical vein endothelial cell
基金项目:国家自然科学基金面上项目(81771604);南京市卫生科技发展专项资金项目(YKK18156)
作者单位
吴莉莉 南京医科大学附属妇产医院产科江苏 南京 210004 
薛 路 南京医科大学附属妇产医院产科江苏 南京 210004 
章 浩 南京医科大学第一附属医院心内科江苏 南京 210029 
乌 兰 南京医科大学附属妇产医院产科江苏 南京 210004 
丁虹娟 南京医科大学附属妇产医院产科江苏 南京 210004 
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中文摘要:
      目的:探究lncRNA HAND反义RNA1(HAND2?AS1)、miRNA hsa?miR?520c?3p和整合素亚基β8(integrin subunit beta 8,ITGB8)在子痫前期患者胎盘中表达量的改变以及HAND2?AS1对血管内皮功能的影响和机制。方法:收集25例子痫前期患者胎盘样本和20例匹配的健康产妇胎盘样本,通过荧光定量PCR技术检测HAND2?AS1、hsa?miR?520c?3p和ITGB8的表达量。通过细胞活力、划痕、血管形成和荧光定量PCR实验,观察过表达和敲低HAND2?AS1对人脐静脉内皮细胞(human umbilical vein endothelial cell,HUVEC)增殖、迁移和血管形成的影响以及对hsa?miR?520c?3p和ITGB8表达量的影响。结果:与对照组胎盘相比,子痫前期患者胎盘中HAND2?AS1和ITGB8的表达量显著升高,而hsa?miR?520c?3p表达量降低。同时,子痫前期患者的胎盘中,HAND2?AS1的表达量与hsa?miR?520c?3p呈负相关,与ITGB8呈正相关。在HUVEC中过表达HAND2?AS1,HUVEC的增殖、迁移和血管形成能力减弱,同时hsa?miR?520c?3p表达量下降,ITGB8表达增高;敲低HAND2?AS1,结果相反。结论:HAND2?AS1抑制血管内皮增殖、迁移和血管形成能力,其潜在机制可能是通过调控hsa?miR?520c?3p/ITGB8的表达,有望成为子痫前期的治疗靶点。
英文摘要:
      Objective:This study aims to investigate the expression patterns of lncRNA HAND2?AS1,miRNA hsa?miR?520c?3p and integrin subunit beta 8(ITGB8) in placentas of preeclampsia(PE) patients and the role of HAND2?AS1 played in modulating vascular endothelial cells. Methods:Total 25 placentas of PE patients and 20 placentas of healthy controls were collected to detect the expressing abundance of HAND2?AS1,hsa?miR?520c?3p and ITGB8 using qRT?PCR technology. Proliferation,migration and angiogenesis of human umbilical vein endothelial cell(HUVEC) was detected through cell viability assay,wound healing assay and tube formation assay,respectively,when HAND2?AS1 was overexpressed or knock?down. Also,expression of hsa?miR?520c?3p and ITGB8 was detected using QPCR. Results:Compared with healthy controls,expression of HAND2?AS1 and ITGB8 increased and expression of hsa?miR?520c?3p decreased significantly in placentas of PE patients. In placentas of PE patients,the expression of HAND2?AS1 was negatively correlated with hsa?miR?520c?3p and positively correlated with ITGB8. The proliferation,migration and angiogenesis of HUVEC were impaired,the expression of hsa?miR?520c?3p decreased and the expression of ITGB8 increased when HAND2?AS1 was overexpressed. The result was the opposite when HAND2?AS1 was knocked?down. Conclusion:HAND2?AS1 inhibits proliferation,migration and angiogenesis of vascular endothelial cells,of which potential mechanism may be modulating expression of hsa?miR?520c?3p/ITGB8.
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