文章摘要
夏 阳,闻 伟.血浆外泌体miR⁃1260b的表达与肺腺癌患者临床特征的相关性[J].南京医科大学学报,2020,(12):1779~1783
血浆外泌体miR⁃1260b的表达与肺腺癌患者临床特征的相关性
Correlation between plasma exosomal miR ⁃ 1260b and the clinical characteristics of patients with lung adenocarcinoma
投稿时间:2020-07-19  
DOI:10.7655/NYDXBNS20201207
中文关键词: 肺腺癌  外泌体  miRNA
英文关键词: lung adenocarcinoma  exosomes  miRNA
基金项目:国家自然科学基金(青年项目)(81702262)
作者单位
夏 阳 南京医科大学第一附属医院胸外科江苏 南京 210029 
闻 伟 南京医科大学第一附属医院胸外科江苏 南京 210029 
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中文摘要:
      目的:探讨血浆外泌体miR?1260b在肺腺癌中的表达及其与临床特征的关系。方法:收集并提取肺腺癌患者血浆中外泌体,通过透射电镜(transmission electron microscopy,TEM)、粒径分析(nanoparticle tracking analysis,NTA)、Western blot鉴定外泌体的大小、分布情况。采用qRT?PCR检测外泌体miR?1260b的表达。结果:外泌体广泛存在于肺腺癌患者血浆中。亚组分析发现,与肿瘤≤3 cm组相比,血浆外泌体miR?1260b在肿瘤>3 cm组中呈高表达(P=0.010);与N0组相比,血浆外泌体 miR?1260b在N1(P<0.05)、N2(P<0.001)组中呈高表达;与非气道播散组相比,血浆外泌体 miR?1260b在气道播散组中高表达(P<0.001)。与未复发/转移组相比,血浆外泌体 miR?1260b在复发/转移组中高表达(P=0.038)。结论:血浆外泌体 miR?1260b在肺腺癌中高表达,且与肿瘤大小、气道播散、复发/转移相关,可作为肺腺癌患者诊治的分子靶标。
英文摘要:
      Objective:This study aimgs to investigate the expression of plasma exosomal miR?1260b in patients with lung adenocarcinoma and its correlation with clinical features. Methods:Exosomes were extracted from the plasma of the patients with lung adenocarcinoma. The size and distribution of exosomes were identified by transmission electron microscopy(TEM),nanoparticle tracking analysis(NTA) and Western blot. qRT?PCR was used to detect the expression of exosomal miR?1260b. Results:Exosomes wre widely present in the plasma of patients with lung adenocarcinoma. Subgroup analysis found that plasma exosomal miR?1260b was highly expressed in> 3 cm compared with ≤ 3 cm group(P=0.010);Compared with the N0 group,plasma exosomal miR?1260b was increased in the N1(P<0.05)and N2(P<0.001)groups;Compared with the spread without air spaces group,plasma exosomal miR?1260b was elevated in the spread through air spaces group(P<0.001). Compared with the non?relapse/metastasis group,plasma exosomal miR?1260b was highly expressed in the relapse/metastasis group(P=0.038). Conclusion:Plasma exosomal miR?1260b is highly expressed in patients with lung adenocarcinoma,and is related to tumor size,stas and recurrence/metastasis. It can be used as a molecular target for the diagnosis and treatment of lung adenocarcinoma patients.
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