文章摘要
Yuefeng Du,Yifei Xing,Fuqing Zeng,Peng Lu,Xianyin Liu.[J].南京医科大学学报,2006,20(2):
The inhibitory effects of siRNA expression vector on CXCR4 expression in prostate carcinoma cell lines
  
DOI:10.7655
中文关键词: 
英文关键词: RNA interference(RNAi)  CXC receptor 4  expression vector  RT-PCR  western blot
基金项目:
Yuefeng Du  Yifei Xing  Fuqing Zeng  Peng Lu  Xianyin Liu
Department of Urology, Union hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Department of Urology, Union hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Department of Urology, Union hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Department of Urology, Union hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China;Department of Urology, Union hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
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英文摘要:
      Objective: To investigate the inhibitory effects of RNAi (RNA interference, RNAi) expression vector on CXCR4expression in prostate carcinoma cell lines. Methods: Small interference RNA (siRNA) expression vectors for CXCR4 gene were constructed and transfected into prostate carcinoma cell lines(PC-3m and LNCaP)with liposomes. The expression of CXCR4 was detected by RT-PCR and western blot. Results: The expression of CXCR4 mRNA and protein in the PC-3m and LNCaP cells was reduced by RNAi expression vectors. The inhibitory rate of CXCR4 mRNA expression in the PC-3m cells was 87.81% ± 10.20% ,56.10% ± 9.32%at the 24th hour and the 48th hour, compared with 56.93% ±8.78% ,49.24% ± 11.23% in LNCaP cells. The inhibitory rate of the expression of CXCR4 protein was 64.71% ± 6.68% ,58.66% ± 11.56% respectively. Conclusion: The expression of CXCR4 gene can effectively he inhibited by RNAi expression vectors.
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