文章摘要
Jie Zhao, Weibin Sun,Juan Wang.[J].南京医科大学学报,2008,28(1):28~33
Preliminary study on Herpes simplex virus type 1 infection of human oral epithelial cell in vitro
投稿时间:2007-08-31  
DOI:10.7655
中文关键词: 
英文关键词: herpes simplex virus type 1  human oral epithelial cells  transmission electron microscope  polymerase chain reaction
基金项目:
作者单位
Jie Zhao Institute of Stomatology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China 
Weibin Sun  
Juan Wang  
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中文摘要:
      
英文摘要:
      Objective: To explore the functions and mechanisms of herpes simplex virus type 1(HSV-1) while infecting human oral epithelial cells in vitro(being similar to the infection in vivo). Methods:An abundance of HSV-1 strains amplified in Vero cells were used to infect human oral epithelial cells. The culture supernatant was collected to infect Vero cells again. Morphology of HSV-1 was identified by inverted microscope and transmission electron microscope. Nucleic acid of the virus was detected by PCR. Results:The infected human oral epithelial cells didn’t display an obvious cytopathic effect(CPE) under inverted microscope(while Vero cells which were infected by the culture supernatant showed typical(CPE). The virus particles were not observed in the cytoplasm nor in nucleus of human oral epithelial cells, however under transmission electron microscope in the cytoplasm of Vero cells, the nucleic acid of HSV-1 could be detected in infected human oral epithelial cells, by PCR. Conclusion:HSV-1 can successfully infect human oral epithelial cells. This model may provide a useful approach for studying the pathogenesis of herpes virus-associated periodontal disease.
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