文章摘要
Ping Yan,Yiqiang Chen,Zhijun Song,Hong Wu,Jinliang Kong,Xuejun Qin.[J].南京医科大学学报,2008,28(1):34~38
Pathogenic effects of biofilm with chronic pseudomonas aeruginosa lung infection in rats
投稿时间:2007-07-23  
DOI:10.7655
中文关键词: 
英文关键词: P. aeruginosa  biofilm  pulmonary infection  pathogenic effect
基金项目:
作者单位
Ping Yan Respiratory Disease Ward, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China 
Yiqiang Chen Respiratory Disease Ward, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China 
Zhijun Song Department of Pathophysiology, Guangxi Medical University, Nanning 530021,Guangxi Province,China 
Hong Wu Department of Clinical Microbiology, University Hospital of Copenhagen, Denmark 
Jinliang Kong Respiratory Disease Ward, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China 
Xuejun Qin Respiratory Disease Ward, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, Guangxi Province, China 
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中文摘要:
      
英文摘要:
       Objective: To establish an animal model of P.aeruginosa biofilm associated with chronic pulmonary infection and investigate the pathogenic effects of biofilm. Methods: Experiments in vitro, measuring the MICS, MBCS of levofloxacin(LFX), ceftazidime(CAZ) in PAO579 in alginate beads and planktonic PAO579. Rats were challenged with 0.1 ml of PAO579(109CFU/ml) in alginate beads or 0.1 ml of planktonic PAO579(109 CFU/ml), 3,7,14 days after challenging, bacteriological, pathological features were observed. Results: The MICS, MBCS of LFX, CAZ in PAO579 in alginate beads were higher than those in planktonic PAO579 in vitro. CFU/lung in alginate beads group was significantly higher than that in planktonic bacteria group(P = 0.002, P = 0.004, P = 0.002, respectively); macroscopic lung pathology and the inflammation in alginate beads group were significantly more severe compared to those in planktonic bacteria group in vivo. Conclusion: P.aeruginosa biofilm protected bacterium from killing of antibiotics and might mediate the host immune damage in the lung tissue and made bacterium evade the host immune defense.
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