文章摘要
Yinxue Song,Jun Gong.[J].南京医科大学学报,2008,28(4):217~223
Effect of bile salts and bile acids on human gastric mucosal epithelial cells
投稿时间:2005-05-22  
DOI:10.7655
中文关键词: 
英文关键词: bile salts  bile acid  duodenogastric reflux  gastric mucosal epithelial cells  apoptosis
基金项目:
作者单位
Yinxue Song Department of Digestive, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China 
Jun Gong  
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英文摘要:
      Objective:To explore the effect of bile salt and bile acid on cultured eternalized human gastric mucosa epithelium GES-1 cells. Methods:Cultured eternalized human gastric mucosa epithelium GES-1 cells were treated with media containing 6 different kinds of bile salts and 3 different kinds of bile acids and their mixture with different concentrations: GCDC(glycochenodeoxycholate), GDC(glycodeoxycholate), GC(glycocholate), TCDC(taurochenodeoxycholate), TDC(taurodeoxycholate), TC (taurocholate), LCA(lithocholicacid), CA(cholic acid), DCA(deoxycholic acid)(50μmol/L,250μmol/L,500μmol/L,1000μmol/L), DY(mixture of bile salts) and DS(mixture of bile acids)(250μmol/L,500μmol/L,1000μmol/L,1500μmol/L, 2000μmol/L), in comparison with the control group(in normal media without bile salts and bile acids). Cell proliferation was assessed by MTT(3-[4,5-Dimethylthiaolyl]-2,5-diphenyl-tetrazolium bromide) assay for 72 hours with different concentrations and the apoptotic cells were assayed by flow cytometry (FCM) with Annex V-FITC conjugated with propidium iodide(PI) staining for 24 hours with different concentrations(1500,2000μmol/L). Results:There was no significant difference in morphology and cell proliferation in GC group after 24~72 h. Low concentration(50μmol/L) of GCDC, GDC, TCDC, TDC and TC accelerated gastric epithelial cell growth in a dosage-time dependent manner. At middle concentration(250-500μmol/L), it showed positive effect after 24~48 h, while negative effect after 72 h. At high concentration(1000μmol/L), it accelerated gastric epithelial cell growth after 24h and show consistent inhibition even leading to necrosis after 48~72 h. LCA and CA showed a positive effect on the concentration of 50μmol/L after 24~72 h, while 250-1000μmol/L showed a trend towards apoptosis after 24~72 h. At 50-500μmol/L, DCA showed proliferation after 24 h and apoptosis after 48~72 h, but showed necrosis after 24~72 h at 1000 μmol/L. DY and DS could facilitate normal gastric mucosa epithelial cell growth at low concentration (250-500μmol/L), however at 1000-2000μmol/L the trend shifted from apoptosis to necrosis. FCM with Annexin-V conjugated with PI staining revealed that GCDC, GDC, GC, TCDC, TDC, TC, LCA, CA, DCA, DY and DS induced apoptosis of human gastric mucosal epithelial cells. They were all significantly higher than that of the control(P < 0.05), but there was no significant difference in GC group (P > 0.05). The bile salts induced apoptosis in a time-dose-dependent manner. Conclusion:Our results suggested that bile acid and bile salt is the trigger of injury in human gastric mucosal epithelial cells.
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