文章摘要
Xiaofeng Chen,Yongqian Shu, Wei Li,Yongmei Yin.[J].南京医科大学学报,2008,28(6):366~371
TNF-alpha-induced metastasis gene changes in MCF-7 cells
投稿时间:2008-09-11  
DOI:10.7655
中文关键词: 
英文关键词: Tumor necrosis factor-α  metastasis  cDNA array
基金项目:
作者单位
Xiaofeng Chen Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu province, China 
Yongqian Shu  
Wei Li  
Yongmei Yin  
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中文摘要:
      
英文摘要:
      Objective: Studies have shown that TNF-αsecreted by tumor cells and macrophages infiltrated into the tumor microenvironment might promote the metastasis of a variety of malignant cancers, including breast cancer. The present study was designed to detect global metastasis-related gene expression changes of MCF-7 cells treated by low dose TNF-α and to further explore the mechanisms by which TNF-αcontributes to metastasis. Methods: MCF-7 cells were cultured and treated with low dose TNF-α(20 ng/ml). cDNA array analysis was applied to detect the metastasis related gene expressions. Results: A total of 36 gene expressions were significantly regulated by TNF-α. Functional analysis indicates that the altered genes belong to different functional group. Most of the genes changed may promote the metastasis of MCF-7 cells while the others may inhibit metastasis. The changes observed in gene expression following TNF-αwere somewhat time dependent. Conclusion: TNF-αcan enhance the invasive ability of MCF-7 cells, partly by regulating a series of metastasis related genes, and these genes may take part in every step of metastasis. Some of the genes deserve further study.
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