文章摘要
Renhu Sun,Jiang Li,Jing Cui,Qing Lv,Xinghua Liu,Guobin Wang.[J].南京医科大学学报,2009,29(1):15~19
Effect of Rapamycin on TGF-β1- induced epithelial-mesenchymal transition in LoVo colonic adenocarcinoma cells
投稿时间:2008-10-27  
DOI:10.7655
中文关键词: 
英文关键词: epithelial-mesenchymal transition (EMT)  Rapamycin  TGF-β1  Snail
基金项目:
作者单位
Renhu Sun Department of general Surgery, Union Hospital, Tong ji Medical College,Huazhong Universty of Science and Technology, Wuhan 430022, China 
Jiang Li  
Jing Cui  
Qing Lv  
Xinghua Liu  
Guobin Wang  
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中文摘要:
      
英文摘要:
      Objective:To investigate the effect of Rapamycin on epithelial-mesenchymal transition (EMT) of LoVo colonic adenocarcinoma cells in vitro. Methods:Cultured LoVo colonic adenocarcinoma cells were divided into three groups: negative control group, EMT-inducing group (TGF-β1) and EMT-interfering group (TGF-β1 plus Rapamycin). E-cadherin expression in LoVo cells was detected by Western Blot, while the expression of vimentin was evaluated through immunocytochemistry. The Snail mRNA in LoVo cells was examined by RT-PCR. Results: TGF-β1 induced LoVo cell switching from polygonal to spindle-shaped. TGF-β1 enhanced the expression of vimentin, but lowered the level of E-cadherin. In contrast, Rapamycin impaired the transition induced by TGF-β1. Rapamycin dramatically abrogated TGF-β1-induced vimentin expression and restored E-cadherin expression in LoVo cells. Rapamycin significantly repressed the up-regulation of Snail mRNA expression induced by TGF-β1. Conclusion: Rapamycin dramatically abrogated TGF-β1 induced Snail mRNA expression in LoVo cells, hence inhibiting the EMT of these cells in vitro.
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