Objective：To investigate the expression of lncRNA BANCR in gastric cancer（GC），and explore the potential biological function and underlying molecular mechanism on cell migration and invasion. Methods：The mRNA expression level of BANCR in GC tissues was detected by quantitative real-time PCR（QPCR）. MKN28 cells was transfected with siRNA against BANCR. Transwell assays was used to detect the ability of cell migration and invasion and QPCR was used to detect the expression of metastasis-related markers. Results：The expression level of BANCR in GC tissues was significantly higher than that in adjacent tissues，which consistent with TCGA data. Down-regulation of BANCR repressed GC cell migration and invasion. Furthermore，the expression level of CDH1 was up-regulated，and the expression level of Vimentin and ZEB1 was down-regulated in BANCR knocking-down cells. Interesting，the expression level of ZEB1 was restored by decreasing miR-203 in BANCR knocking-down cells. Conclusion：BANCR was over-expressed in GC tissues and play an oncogenic role by regulating the miR-203-ZEB1-CDH1 axis in GC.