文章摘要
刘媛媛,宋小玲,王仿竹,齐美杰,沈 彬.m6A识别蛋白Ythdf1在精子发生中的作用研究[J].南京医科大学学报,2019,(2):171~175
m6A识别蛋白Ythdf1在精子发生中的作用研究
Effects of m6A reader Ythdf1 during spermatogenesis
投稿时间:2018-09-07  
DOI:10.7655/NYDXBNS20190202
中文关键词: CRISPR/Cas9  Ythdf1  精子发生
英文关键词: CRISPR/Cas9  Ythdf1  spermatogenesis
基金项目:国家自然科学基金(31622039);江苏省自然科学基金(BK20160045)
作者单位
刘媛媛 南京医科大学生殖国家重点实验室江苏 南京 211166 
宋小玲 南京医科大学生殖国家重点实验室江苏 南京 211166 
王仿竹 南京医科大学生殖国家重点实验室江苏 南京 211166 
齐美杰 南京医科大学生殖国家重点实验室江苏 南京 211166 
沈 彬 南京医科大学生殖国家重点实验室江苏 南京 211166 
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中文摘要:
      目的:构建YTH N6甲基腺苷RNA结合蛋白1(YTH N6?methyladenosine RNA binding protein 1,Ythdf1)的基因敲除小鼠,研究其在小鼠精子发生中的作用。方法:利用CRISPR/Cas9敲除技术及原核注射技术构建Ythdf1基因敲除小鼠模型,通过免疫荧光、HE染色对敲除小鼠睾丸及附睾进行形态学分析,通过交配实验进行生殖力测试,研究Ythdf1基因对雄性小鼠精子发生的影响。结果:RT?PCR结果显示,Ythdf1在1~8周龄雄鼠睾丸中均有表达;成功构建Ythdf1基因敲除小鼠;成年敲除小鼠精子发生、附睾及生育力均无异常。结论:在正常饲养情况下,敲除Ythdf1基因对雄性小鼠精子发生无影响。
英文摘要:
      Objective:Construct YTH N6?methyladenosine RNA binding protein 1(Ythdf1) knockout mice and study its role during mouse spermatogenesis. Methods:Ythdf1 knockout mice were generated by microinjection of CRISPR/Cas9 into zygote successfully. The role of Ythdf1 in mouse spermatogenesis was detected by immunofluorescence,HE and fertility test. Results:The results of RT?PCR showed that Ythdf1 was expressed in the testis of 1 to 8?week?old male mice. Ythdf1 knockout mice were successfully generated. These knockout mice showed normal spermatogenesis,epididymis morphology and fertility. Conclusion:This study found that deletion of Ythdf1 gene had no effect on spermatogenesis under normal breeding conditions.
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