文章摘要
周婷婷,朱 进.新型冠状病毒实验室检测方法及应用[J].南京医科大学学报,2020,(4):474~477
新型冠状病毒实验室检测方法及应用
Application and laboratory detection of severe acute respiratory syndrome coronavirus⁃2
投稿时间:2020-03-09  
DOI:10.7655/NYDXBNS20200403
中文关键词: 新型冠状病毒  新型冠状病毒肺炎  基因组测序  免疫学诊断
英文关键词: SRAS⁃CoV⁃2  COVID⁃19  genome sequencing  immunological diagnosis
基金项目:
作者单位
周婷婷 东部战区疾病预防控制中心江苏 南京 210002 
朱 进 东部战区疾病预防控制中心江苏 南京 210002 
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中文摘要:
      随着新型冠状病毒(severe acute respiratory syndrome coronavirus?2,SRAS?CoV?2)分离培养与基因组测序的完成,新型冠状病毒肺炎(corona virus disease,COVID?19)的确诊主要依靠RT?PCR 法检测SARS?CoV?2 核酸基因,重组酶介导等温核酸扩增技术(recombinase aided amplification,RAA)等新型常温核酸扩增技术也得到进一步应用。免疫学诊断包括胶体金和ELISA IgM 和IgG 抗体检测技术,主要用于人群的筛查和辅助诊断。基于CRISPR技术的新型诊断技术等也在进一步完善过程中。
英文摘要:
      After the completion of the severe acute respiratory syndrome coronavirus?2 (SARS?CoV?2) isolation,culture and genome sequencing,the diagnosis of new coronavirus pneumonia mainly depends on the detection of the SARS?CoV?2 nucleic acid by RT?PCR. In addition,the new room temperature nucleic acid amplification technologies such as recombinase aided amplification(RAA)have been further applied in lab detection. Immunological diagnosis includes colloidal gold and IgM/IgG antibody detection by ELISA technology,which is used for screening and auxiliary diagnosis of the population. New diagnostic technologies based on CRISPR are also in the process of further improvement.
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